17 September 2019 : Laboratory Research
Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical LesionsShuang Sui1B, Hongxiang Chen1C, Lili Han1E, Lin Wang1A*, Mayineur Niyazi1AD, Kaichun Zhu1F
Med Sci Monit 2019; 25:6990-6997
BACKGROUND: We studied the effect of APOBEC3G on persistent human papillomavirus (HPV) infection and the correlation between APOBEC3G polymorphism and HPV persistent infection and cervical disease progression in Uygur women in China.
MATERIAL AND METHODS: From January 2015 to December 2017, we enrolled 529 Uygur ethnic group patients with HPV infection. SIHA cells were transfected with APOBEC3G. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were used to detect mRNA and protein expression levels of APOBEC3G and HPV E6 and p53. Exon 3 of APOBEC3G was sequenced by first-generation sequencing.
RESULTS: The mRNA and protein expression levels of APOBEC3G in the cervical cancer group were significantly higher than in the cervical intraepithelial neoplasia (CIN) group (p<0.05). The mRNA and protein expression levels of APOBEC3G in the CIN group were significantly higher than in the non-cervical lesions group (p<0.05). The mRNA and protein expression levels of HPV E6 in SIHA cells transfected with APOBEC3G were significantly lower than in the control group and the no-load group (p<0.05), and the mRNA and protein expression levels of p53 were significantly higher than in the control group and the no-load group (p<0.05). There was a polymorphic locus rs5757465 on exon 3 of APOBEC3G in Uygur women, and this rare CC type was a risk factor for cervical lesions and cervical cancer (OR=3.714, 95%CI: 1.916–7.202, p<0.05).
CONCLUSIONS: APOBEC3G is involved in continuous HPV infection, cervical prelesions, and the development of cervical cancer, and the rare genotype (CC) of APOBEC3G may be one of the factors causing cervical lesions in Uygur women who have HPV infection.
Keywords: Human papillomavirus 16, Polymorphism, Genetic, Uterine Cervical Neoplasms, APOBEC-3G Deaminase, Alleles, Base Sequence, Disease Progression, Exons, Gene Expression Regulation, Neoplastic, Genetic Loci, Genetic Predisposition to Disease, Papillomavirus Infections, Polymorphism, Single Nucleotide, RNA, Messenger, Tumor Suppressor Protein p53
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