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04 September 2019 : Laboratory Research  

α-Pinene Induces Apoptotic Cell Death via Caspase Activation in Human Ovarian Cancer Cells

Jing Hou1ABCDEF, Yi Zhang2ABC, Yanju Zhu1ACF, Bingfeng Zhou1BCF, Chunli Ren1ACF, Shuang Liang1ADF, Yanwei Guo1ABCDEFG*

DOI: 10.12659/MSM.916419

Med Sci Monit 2019; 25:6631-6638


BACKGROUND: The plant-derived terpenoid, α-pinene is a bicyclic monoterpene potentially useful for the treatment of various diseases which also includes cancer and its types. The present investigation is about finding the anticancer activity of the α-pinene extracted from the leaves of Boswellia dalzielii over the PA-1 cancer cells of the human ovary.

MATERIAL AND METHODS: The cytotoxic activity of the α-pinene was evaluated using MTT and LDH assays which indicated that α-pinene could induce cytotoxicity in cancer-causing cells in the ovary. The consequences of α-pinene on the cell sequence regulation were determined by the staining technique using propidium iodide (PI) followed with flow cytometry.

RESULTS: The cell cycle distribution analysis showed that α-pinene inhibit the cycle progression from G₂ to M phase. In addition, apoptosis analysis is done through the double staining investigation using Annexin V-FITC/PI to analyze the controlled growth of α-pinene which is associated with the apoptosis. Caspase-3 a crucial enzyme involved in apoptosis was markedly increased in the a-pinene treated PA-1 cells. The apoptosis results reveal, that the cancer cells at the human ovary with α-pinene induces the significant populations of apoptotic cells.

CONCLUSIONS: Overall, α-pinene may exert anticancer effects in PA-1 cells by promoting cytotoxicity, suppression of cell sequence progression along with the programmed cell death.

Keywords: Cytotoxicity Tests, Immunologic, Flow Cytometry, Ovarian Neoplasms, Bicyclic Monoterpenes, Boswellia, Cell Cycle, Enzyme Activation, L-Lactate Dehydrogenase, Plant Leaves

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Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.942244

Med Sci Monit 2023; 29:e942244


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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750