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04 June 2019 : Animal Research  

Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs

Deng-Shen Zhang1ABCDEFG, Gui-You Liang1ADG*, Da-Xing Liu1BDF, Jie Yu2BCD, Feng Wang1DEF

DOI: 10.12659/MSM.916517

Med Sci Monit 2019; 25:4149-4158


BACKGROUND: The aim of this study was to determine the role of AMP-activated protein kinase (AMPK) in myocardial insulin resistance after myocardial ischemia-reperfusion during cardiopulmonary bypass surgery in dogs.

MATERIAL AND METHODS: Twenty-four mongrel dogs were randomly assigned to 4 groups. The control group did not undergo aortic cross-clamping; the model group underwent 60 mins of aortic cross-clamping with 150 ml cardioplegic solution. The treatment group, the inhibition group respectively with 0.11mg/kg AICAR (AMPK agonist) in 150 ml cardioplegic solution and 0.11mg/kg Compound C (AMPK inhibitor) in 150 ml cardioplegic solution. The blood flow was determined and left ventricular myocardial tissue were taken at pre-bypass, 15, 60, and 90 min after aorta declamping, respectively. Expression of AMPK mRNA, p-AMPK and GLUT-4 proteins was determined by RT-PCR, IHC and WB.

RESULTS: Compared with the control group, receiving 60 min ischemia at 15 min after reperfusion, Myocardial Glucose Extraction Ratio were significantly decreased in the other 3 groups, it was significantly decreased from 20.0% to 1.2% at 60 min of reperfusion, and recovered to 6.1% after 90 min reperfusion in model group, while recovered to 4.1%, 12.0% after 90 min reperfusion respectively exposed to Compound C and AICAR. The expressions of p-AMPK, GLUT-4 protein and AMPK mRNA in myocardium were decreased in different experiment groups, but these changes occurred to a lesser extent in the treatment group.

CONCLUSIONS: The inability of GLUT-4 expression induced by the decreases in p-AMPK protein expression that may be one of the reasons for myocardial insulin resistance.

Keywords: Cardiopulmonary Bypass, Insulin Resistance, Myocardial Reperfusion Injury, AMP-Activated Protein Kinases, Aminoimidazole Carboxamide, Cardioplegic Solutions, China, Coronary Artery Disease, Dogs, Glucose, Glucose Transporter Type 4, Heart Ventricles, Ischemia, Myocardial Ischemia, Myocardial Reperfusion, Myocardium, Phosphorylation, Pyrazoles, Pyrimidines, Ribonucleotides



01 August 2022 : Editorial  

Editorial: Long-Term Effects of Symptomatic and Asymptomatic SARS-CoV-2 Infection in Children and the Changing Pathogenesis of Common Childhood Viruses Driven by the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.937927

Med Sci Monit 2022; 28:e937927

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750