10 November 2019 : Laboratory Research
Elevated Uric Acid Levels Promote Vascular Smooth Muscle Cells (VSMC) Proliferation via an Nod-Like Receptor Protein 3 (NLRP3)-Inflammasome-Dependent Mechanism
Hui Li1BCDE, Fudong Qian1BEF, Heyu Liu1EF, Zhiyong Zhang2ABCDEF*DOI: 10.12659/MSM.916667
Med Sci Monit 2019; 25:8457-8464
Abstract
BACKGROUND: Hyperuricemia has a pathogenic role in the development of hypertension and other cardiovascular diseases (CVD). Uric acid has been reported to activate Nod-like receptor protein 3 (NLRP3)-inflammasome and alter vascular smooth muscle cells (VSMC). However, the potential mechanisms underlying this association are still not understood. The aim of this study was to investigate the role and potential mechanisms of uric acid in proliferation of VSMC.
MATERIAL AND METHODS: Cell Counting Kit-8 (CCK-8) proliferation assay and colony formation assay were performed to determine the proliferative ability of VSMC under uric acid stimulation. Immunofluorescence microscopy was carried out to determine the expression of Alpha-smooth muscle actin (α-SMA). In addition, real-time PCR and Western blot were used to detect the expression of NLRP3-inflammasome, and ELISA was performed to measure the levels of IL-18 and IL-1β.
RESULTS: The results showed that uric acid increases the proliferation of VSMC and induces α-SMA accumulation. We also found that uric acid increases the level of NLRP3 and induces NLRP3-inflammasome activation. The expressions of uric acid-induced inflammatory markers IL-1β and IL-18 were decreased by the inhibitor MCC950.
CONCLUSIONS: Our findings revealed that uric acid induces inflammation through NLRP3-inflammasome-mediated VSMC proliferation. NLRP3 may be a new therapeutic target for hypertension.
Keywords: hyperuricemia, Muscle, Smooth, Vascular, Nod Signaling Adaptor Proteins, Uric Acid, Actins, Cardiovascular Diseases, Carrier Proteins, Hypertension, Inflammasomes, Interleukin-18, Myocytes, Smooth Muscle, NLR Family, Pyrin Domain-Containing 3 Protein
Editorial
01 September 2024 : Editorial
Editorial: Reasons for Increasing Global Concerns for the Spread of MpoxDOI: 10.12659/MSM.946343
Med Sci Monit 2024; 30:e946343
In Press
Review article
Pharmacological Strategies in Dermatomyositis: Current Treatments and Future DirectionsMed Sci Monit In Press; DOI: 10.12659/MSM.944564
Clinical Research
Effect of Hyaluronic Acid on Socket Healing After Lower Impacted Third Molar Tooth Extraction in 40 Dental ...Med Sci Monit In Press; DOI: 10.12659/MSM.945386
Review article
Cariprazine in Psychiatry: A Comprehensive Review of Efficacy, Safety, and Therapeutic PotentialMed Sci Monit In Press; DOI: 10.12659/MSM.945411
Clinical Research
Influenza Hemagglutinin Antibody Levels in the Elderly: Impact of Sex, Age, and Influenza/COVID-19 Vaccinat...Med Sci Monit In Press; DOI: 10.12659/MSM.945002
Most Viewed Current Articles
17 Jan 2024 : Review article 6,049,426
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research 1,832,003
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research 692,876
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
07 Jan 2022 : Meta-Analysis 257,339
Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...DOI :10.12659/MSM.935074
Med Sci Monit 2022; 28:e935074