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04 July 2019 : Clinical Research  

A New Insight of Kartogenin Induced the Mesenchymal Stem Cells (MSCs) Selectively Differentiate into Chondrocytes by Activating the Bone Morphogenetic Protein 7 (BMP-7)/Smad5 Pathway

Qi Zhou1ACG, Jie-Hong Zhang1BCD, Shuai Yuan1EF, Jia-Hua Shao1DF, Zhu-Yun Cai1CDF, Shu Chen1BDE, Jia Cao1BEF, Hai-Shan Wu1BCE, Qi-Rong Qian1ADF*

DOI: 10.12659/MSM.916696

Med Sci Monit 2019; 25:4960-4967

Abstract

BACKGROUND: Rotator cuff injury is the most common cause of shoulder disability, and although the repair technique has improved, the rate of rotator cuff reduction after repair is still high. The fibrocartilage region, which appears to be histologically inserted, cannot be regenerated. In recent years, studies have reported that mesenchymal stem cells (MSCs) have enhanced cartilage regeneration in the tendon and bone interface after rotator cuff repair, which has become a hot topic of research.

MATERIAL AND METHODS: Two mesenchymal stem cell types, SMSC (synovial-derived mesenchymal stem cells) and BMSC (bone marrow-derived mesenchymal stem cells) were intervened using kartogenin (KGN). The cytotoxicity was evaluated and the proliferation of the 2 cells was observed. Four commonly used cartilage phenotype genes were detected by quantitative real-time polymerase chain reaction, and the cartilage differentiation of MSCs induced by KGN was explored. The bidirectional regulation of the expression of BMP-7 and the downstream gene Smad5 was observed by constructing a lentiviral overexpression vector containing the target gene BMP-7. To explore whether BMP-7/Smad5 pathway activation promotes differentiation of SMSCs into chondrocytes.

RESULTS: KGN can induce the selective differentiation of endogenous MSCs into chondrocytes by activating the BMP-7/Smad5 pathway, which promotes the regeneration of interfacial cartilage, and improves the quality of tendon healing of the tendon after rotator cuff repair.

CONCLUSIONS: This study found a new biological intervention method to promote the effect of tendon on bone healing after rotator cuff repair.

Keywords: Abnormal Karyotype, Cartilage, mesenchymal stromal cells, rotator cuff, Anilides, Bone Morphogenetic Protein 7, Cell Differentiation, Cell Shape, Chondrocytes, HEK293 Cells, Phthalic Acids, Smad5 Protein, Synovial Membrane

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750