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07 September 2019 : Animal Research  

Antagonistic Effect of Cuscuta chinensis on a Rat Model with Unilateral Cryptorchidism

Daorui Qin12AE*, Yunman Tang2BF, Xuejun Wang2CF, Yu Mao2CG, Zhichun Feng13BD

DOI: 10.12659/MSM.916893

Med Sci Monit 2019; 25:6727-6735


BACKGROUND: The aim of this study was to investigate the effect of Cuscuta chinensis Lam. on germ cell apoptosis in a rat model of unilateral cryptorchidism.

MATERIAL AND METHODS: Thirty male SD rats were randomly and equally divided into a control group, a model group, and a Cuscuta group (5.0 g/kg/d) (n=10). The rat model of unilateral cryptorchidism in the model and Cuscuta groups was established by removal of the right gubernaculum, while rats in the control group received no treatment. After modeling, rats in the Cuscuta chinensis group were intragastrically administered Cuscuta chinensis extract (5.0 g/kg/d), while rats in the control group and model group were administered an equal volume of normal saline. After 90 days, all the rats were sacrificed and the testicles were separated and weighed, followed by TUNEL staining to detect germ cell apoptosis, flow cytometry to measure JC-1, ROS, and MDA, and Western blot analysis to evaluate the expression of Bax, Bcl-2, and cleaved caspase3.

RESULTS: Ninety days after the operation, Cuscuta chinensis Lam significantly minimized the damage caused by modeling by increasing weight of testis, reducing the germ cell apoptosis, and enhancing the mitochondrial membrane potential of testicles, as shown by levels of JC-1, ROS, and MDA, as well as elevating the level of Bcl-2/Bax and reducing the level of cleaved caspase3 (P<0.05).

CONCLUSIONS: Treatment with Cuscuta chinensis Lam reduced the germ cell apoptosis in rats with unilateral cryptorchidism, which provides new insight for the development of cryptorchidism therapy in the future.

Keywords: Cryptorchidism, Neoplasms, Germ Cell and Embryonal, Cuscuta, Malondialdehyde, Membrane Potential, Mitochondrial, Organ Size, Phytotherapy, Plant Extracts, Spermatozoa, Testis, bcl-2-Associated X Protein

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01 September 2023 : Editorial  

Editorial: A Rapid Global Increase in COVID-19 is Due to the Emergence of the EG.5 (Eris) Subvariant of Omicron SARS-CoV-2

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.942244

Med Sci Monit 2023; 29:e942244


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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750