Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

26 July 2019 : Laboratory Research  

Small Nucleolar RNA Host Gene 12 (SNHG12) Promotes Proliferation and Invasion of Laryngeal Cancer Cells via Sponging miR-129-5p and Potentiating WW Domain-Containing E3 Ubiquitin Protein Ligase 1 (WWP1) Expression

Jing Li1ABCF, Suguang Sun1BCE, Wei Chen1ABCDEFG*, Kun Yuan1E

DOI: 10.12659/MSM.917088

Med Sci Monit 2019; 25:5552-5560

Abstract

BACKGROUND: The clinical significance and biological function of long noncoding RNA SNHG12 have not been identified in laryngeal squamous cell carcinoma (LSCC).

MATERIAL AND METHODS: Expression levels of SNHG12, miR-129-5p, and WWP1 in LSCC tissues or cells were tested by RT-qPCR. MTT assay, flow cytometry, and Transwell assay were used to identify the progression of LSCC cells in vitro. Luciferase reporter assay was used to assess the associations among SNHG12, WWP1, and miR-129-5p.

RESULTS: SNHG12 was significantly overexpressed in LSCC tissues compared with adjacent normal tissues. The expression level of SNHG12 was significantly associated with T classification, lymph node metastasis, and cancer stage of LSCC. High expression of SNHG12 predicted shorter disease-free survival. Suppressing SNHG12 using siRNA inhibited proliferation and invasion and promoted apoptosis in the AMC-HN-8 LSCC cell line. SNHG12, mainly located in cytoplasm of AMC-HN-8 cells, was validated by dual luciferase reporter test and RT-qPCR to directly interact with miR-129-5p. Inhibition of miR-129-5p significantly increased proliferation and invasion of AMC-HN-8 cells and ameliorated the suppressive effects of si-SNHG12. Luciferase assay showed that miR-129-5p was able to combine with the 3’UTR region of WWP1, which is generally regarded as an E3 ubiquitin protein ligase. RT-qPCR and Western blot showed that WWP1 was positively regulated by SNHG12 and negatively regulated by miR-129-5p at the mRNA level and protein level. Overexpression of WWP1 significantly increased proliferation and invasion of laryngeal cancer cells. Moreover, when SNHG12 was suppressed, rescue of WWP1 restored the proliferation and invasion abilities of AMC-HN-8 cells.

CONCLUSIONS: Our study demonstrated that SNHG12 promoted LSCC cells progression via sponging miR-129-5p and potentiating WWP1 expression.

Keywords: Laryngeal Neoplasms, RNA, Long Noncoding, Aged, Gene Expression, Middle Aged, Neoplasm Invasiveness, Prognosis, Squamous Cell Carcinoma of Head and Neck, transcriptome, Ubiquitin-Protein Ligases

Add Comment 0 Comments

1206 14

Editorial

01 November 2023 : Editorial  

Editorial: Factors Driving New Variants of SARS-CoV-2, Immune Escape, and Resistance to Antiviral Treatments as the End of the COVID-19 Pandemic is Declared

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville,

DOI: 10.12659/MSM.942960

Med Sci Monit 2023; 29:e942960

0:00

In Press

30 Nov 2023 : Review article  

Decoding the Neurological Sequelae of General Anesthesia: A Review

Med Sci Monit In Press; DOI: 10.12659/MSM.942740  

30 Nov 2023 : Clinical Research  

Enhanced Pain Relief and Muscle Growth in Individuals with Low Back Instability: The Impact of Blood Flow R...

Med Sci Monit In Press; DOI: 10.12659/MSM.942508  

29 Nov 2023 : Clinical Research  

Comparative Analysis of Intramedullary Nail versus Plate Fixation for Fibula Fracture in Supination Externa...

Med Sci Monit In Press; DOI: 10.12659/MSM.941909  

29 Nov 2023 : Clinical Research  

Impact of COVID-19 on Academic Burnout among Medical College Students in China: Findings from a Web-Based S...

Med Sci Monit In Press; DOI: 10.12659/MSM.942317  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

0:00

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750