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26 October 2019 : Clinical Research  

Long Non-Coding RNA (LncRNA) CAIF is Downregulated in Osteoarthritis and Inhibits LPS-Induced Interleukin 6 (IL-6) Upregulation by Downregulation of MiR-1246

Ke Qi1ABCDE, Rongqiang Lin1BCDE, Chenchen Xue1BC, Tianze Liu1BC, Yiming Wang1B, Yongjin Zhang1ABCEG*, Jia Li1ABEFG

DOI: 10.12659/MSM.917135

Med Sci Monit 2019; 25:8019-8024

Abstract

BACKGROUND: Osteoarthritis (OA) affects about 40% of people older than 40 years of age, and the mechanism is not well understood. Long non-coding RNA (lncRNA) CAIF is a recently identified critical player in myocardial infarction, while its role in other human diseases is unclear. The present study aimed to investigate the role of CAIF in OA.

MATERIAL AND METHODS: Levels of CAIF in synovial fluid of OA patients (n=60) and healthy controls (n=60) were measuring by performing quantitative real-time polymerase chain reaction (qRT-PCR). MiR-1246 and interleukin (IL)-6 levels in synovial fluid were measured by performing qRT-PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Cell apoptosis analysis was performed after CHON-001 cells were treated with 500 mg/mL lipopolysaccharide (LPS) for 24 hours.

RESULTS: We found that CAIF in synovial fluid was downregulated in OA patients and inversely correlated with miR-1246 and IL-6. Downregulated CAIF distinguished OA patients from healthy controls. In cells of chondrogenic cell line CHON-001, CAIF overexpression mediated the inhibited expression of miR-1246 and secretion of IL-6, while miR-1246 overexpression reduced the effects of CAIF overexpression on IL-6 secretion. In addition, CAIF overexpression inhibited the apoptosis of CHON-001 cells under LPS treatment, while miR-1246 overexpression attenuated the effects of CAIF overexpression.

CONCLUSIONS: Therefore, CAIF may downregulate miR-1246 to improve OA.

Keywords: Apoptosis, Osteoarthritis, RNA, Long Noncoding, Aged, Case-Control Studies, Cell Line, Chondrocytes, Interleukin-6, Lipopolysaccharides, MicroRNAs, Middle Aged, Synovial Fluid, Up-Regulation

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750