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01 November 2019 : Animal Research  

Nuclear Factor kappa B (NF-κB) Targeted Self-Assembled Nanoparticles Loaded with Methotrexate for Treatment of Rheumatoid Arthritis

Xiong Li1AG*, Jin Qu1EF, Tao Zhang1B, Xi He1BC, Ying Jiang2D, Jiangyan Chen2E

DOI: 10.12659/MSM.917396

Med Sci Monit 2019; 25:8204-8212

Abstract

BACKGROUND: Nanotechnology is one of the most productive approaches for specifically delivering drug payloads to the region of interest to decrease nonspecific distribution and unwanted toxicities.

MATERIAL AND METHODS: We prepared glycol chitosan stearate self-assembled nanoparticles loaded with methotrexate (MTX) for NF-κB targeting in treatment of rheumatoid arthritis (RA). The nanoparticles were prepared using hydrophobic modification of glycol chitosan (GC) with steric acid (SA) and was characterized using IR. The efficiency of nanoparticles after their physiochemical characterization was measured in vitro and by in vivo studies in mice.

RESULTS: The nanoparticles thus prepared were spherical in shape, 235 nm in diameter, and had negative zeta potential. The entrapment efficiency of MTX-GC-SA was more than 70%. The in vitro higher uptake of MTX-GC-SA in murine macrophage cells (RAW 264.7) was confirmed using confocal microscopy and FACS analysis. Systemic administration of MTX-GC-SA into collagen-induced arthritis (CIA) mice resulted in high accumulation in inflamed joints. The MTX-GS-SA revealed significantly better therapeutic efficacy against CIA mice compared to free MTX.

CONCLUSIONS: These findings highlight the potential of using this MTX-GC-SA nanoparticle formulation in suppressing inflammatory arthritis for effective treatment of RA.

Keywords: Arthritis, Rheumatoid, Methotrexate, Nanoparticles, Antirheumatic Agents, Arthritis, Experimental, Chitosan, Drug Carriers, Particle Size, RAW 264.7 Cells

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Editorial

01 October 2022 : Editorial  

Editorial: Rebound COVID-19 and Cessation of Antiviral Treatment for SARS-CoV-2 with Paxlovid and Molnupiravir

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.938532

Med Sci Monit 2022; 28:e938532

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750