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16 December 2019 : Laboratory Research  

Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis

Zheng Chen1BC, Zhen Zhang2CD, Dongbo Zhao1CD, Wei Feng1DE, Fanlai Meng3EF, Shihui Han1DE, Bin Lin1CD, Xin Shi1AG*

DOI: 10.12659/MSM.918230

Med Sci Monit 2019; 25:9618-9629

Abstract

BACKGROUND: The aim of this study was to investigate the effects and mechanisms of long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development.

MATERIAL AND METHODS: Collecting the 3 pairs of adjacent and hepatocellular carcinoma tissue and analysis by gene chip. Evaluating the FOXD2-AS1 expression by in situ hybridization assay. Evaluating the FOXD2-AS1 to Bel-7402 biological activity in vitro study by Cell Counting Kit-8, flow cytometry, Transwell and wound healing assay and correlation between miR-185 by dual-luciferase reporter assay. The relative proteins expressions were evaluated by western blot assay.

RESULTS: FOXD2-AS1 was significantly upregulation in hepatocellular carcinoma tissues. FOXD2-AS1 knockdown suppressed Bel-7401 cell biological activities (proliferation, invasion, and migration) with miR-185 overexpression and AKT depressing in cell expression.

CONCLUSIONS: LncRNA FOXD2-AS1 promoted hepatocellular carcinoma development by regulation miR-185/AKT axis.

Keywords: Biological Transport, Active, Proto-Oncogene Proteins c-akt, RNA, Long Noncoding, Base Sequence, Carcinoma, Hepatocellular, Cell Nucleus, Gene Expression Regulation, Neoplastic, Hepatocytes, Liver Neoplasms, Middle Aged, Neoplasm Invasiveness, Neoplasm Metastasis, Phosphorylation, RNA, Messenger

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750