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24 December 2019 : Animal Research  

Serum Proteomic Profiling Analysis of Rats Chronically Exposed to Arsenic

Ling Zhang1ABCG*, Jia Huang12BCD, Qin Lin1BE, Yan Ma2CF, Rongxiang Xia1BC, Yuming Zhu1BF, Saimaitikari Abudubari2CE

DOI: 10.12659/MSM.918696

Med Sci Monit 2019; 25:9923-9932

Abstract

BACKGROUND: Arsenic (As) is an environmental contaminant, and As pollution in water and soil is a public health issue worldwide. As exposure is associated with the incidence of many disorders, such as arteriosclerosis, diabetes, neurodegenerative diseases, and renal dysfunction. However, the mechanism of As toxicity remains unclear.

MATERIAL AND METHODS: We investigated the changes in serum protein profiles of rats chronically exposed to As. Twenty healthy rats were randomly divided into 4 groups, and sodium arsenite of varying final concentrations (0, 2, 10, and 50 mg/L, respectively) was add into the drinking water for each group. The administration lasted for 3 months. Two proteomic strategies, isobaric tags for relative and absolute quantitation (iTRAQ), and 2-dimensional gel electrophoresis (2-DE), were employed to screen the differential serum proteins between control and arsenite exposure groups.

RESULTS: We identified a total of 27 differentially-expressed proteins, among which 9 proteins were significantly upregulated and 18 were downregulated by As exposure. Many of the differentially-expressed proteins were related to fat digestion and absorption, including 5 apolipoproteins, which indicated lipid metabolism may be the most affected by As exposure.

CONCLUSIONS: This study revealed the influence of As on lipid metabolism, suggesting an increased potential risk of relevant diseases in subjects chronically exposed to As.

Keywords: Arsenic, Electrophoresis, Gel, Two-Dimensional, Proteomics, Rats, Inbred Strains, Arsenic Poisoning, Arsenites, Gene Expression Profiling, Lipid Metabolism, Proteins, Sodium Compounds

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750