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07 December 2019 : Animal Research  

An Efficient Schistosoma japonicum Bivalent Membrane Protein Antigen DNA Vaccine Against Schistosomiasis in Mice

Na Lei12BCEG, Feng-Chun Liu1BCDE, Cui-Ping Ren1BCF, Ji-Jia Shen1AFG, Miao Liu1AEG*

DOI: 10.12659/MSM.919195

Med Sci Monit 2019; 25:9319-9326


BACKGROUND: Schistosomiasis is one of the most important infectious parasitic diseases in the world. The most important was to control schistosomiasis is through a combination of medical therapy and immunization. The membrane antigens Tsp2 and 29 from Schistosoma are promising anti-schistosomiasis vaccine candidates.

MATERIAL AND METHODS: In this study, the pcDNA3.1(+)-SjTsp2, pcDNA3.1(+)-Sj29, and pcDNA3.1 (+)-SjTsp2-29 eukaryotic expression vectors were successfully constructed as DNA vaccines, and the protective abilities of these vaccines were evaluated in mice.

RESULTS: The results showed that vaccination with SjTsp2, Sj29, and SjTsp2-29 reduced parasite burden and hepatic pathology compared to the control group, and the protective effect of the bivalent SjTsp2-29 DNA vaccine was better than that of the univalent SjTsp2 or Sj29 DNA vaccines. We also found high levels of IgG, IgG1, and IgG2a against SjTsp2, Sj29, and SjTsp2-29 DNA vaccines, with high expression of IFN-γ and no IL-4 in the mice.

CONCLUSIONS: The double-membrane antigen DNA vaccine SjTsp2-29 elicited protection against Schistosoma infection and might serve as a vaccine candidate.

Keywords: DNA, Schistosoma japonicum, Vaccine Potency, Antibodies, Helminth, Immunization, Membrane Proteins, Mice, Inbred Strains, Schistosomiasis, Thrombospondins, Vaccination, Vaccines, DNA

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Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory Infections

Dinah V. Parums

DOI: 10.12659/MSM.943312

Med Sci Monit 2023; 29:e943312


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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750