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03 February 2020 : Laboratory Research  

MiR-323-3p Targeting Transmembrane Protein with EGF-Like and 2 Follistatin Domain (TMEFF2) Inhibits Human Lung Cancer A549 Cell Apoptosis by Regulation of AKT and ERK Signaling Pathways

Ji-min Fan1ACE, Zheng-rong Zheng2ACEF, Yi-Ming Zeng1BCF, Xiao-yang Chen1BCF*

DOI: 10.12659/MSM.919454

Med Sci Monit 2020; 26:e919454


BACKGROUND: Non-small-cell lung cancer (NSCLC) is predominant and has low 5-year relative survival rate. Therefore, the mechanisms of NSCLC tumorigenesis must be comprehensively elucidated. MicroRNA-323-3p (miR-323-3p) has been widely explored and found to exert functions in tumorigenesis of several cancer types. However, the expression pattern and biological function of miR-323-3p and the molecular mechanism underlying NSCLC development and progression remain unclear.

MATERIAL AND METHODS: Quantitative reverse-transcription polymerase chain reaction was used to detect the expression of miR-323-3p and TMEFF2 in NSCLC cell lines (A549, NCI-H3255, and H1299) and normal cell line (BEAS-2B). Methylthiazolyl tetrazolium, colony formation, and flow cytometry assays were performed to evaluate the effects of miR-323-3p and TMEFF2 on cell proliferation. Transwell assay was conducted to determine the effects of TMEFF2 on cell migration and invasion. Dual-luciferase reporter assay was used to verify whether TMEFF2 is a target of miR-323-3p. Western blot analysis was performed to analyze protein expression.

RESULTS: The expression of miR-323-3p increased in the 3 NSCLC cell lines (A549, NCI-H3255, and H1299). miR-323-3p regulated cellular progression by directly suppressing TMEFF2 expression and indirectly prohibited the activation of AKT and ERK pathways in NSCLC.

CONCLUSIONS: Overall, miR-323-3p was considered a lung cancer oncogene and could be a valuable target for NSCLC therapy.

Keywords: Cellular Microenvironment, A549 cells, Down-Regulation, Gene Expression Regulation, Neoplastic, Membrane Proteins, Neoplasm Invasiveness, Neoplasm Proteins, Proto-Oncogene Proteins c-akt, Up-Regulation



01 January 2023 : Editorial  

Editorial: Current Status of Two Adjuvanted Malaria Vaccines and the World Health Organization (WHO) Strategy to Eradicate Malaria by 2030

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939357

Med Sci Monit 2023; 29:e939357


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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750