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06 April 2020 : Animal Research  

Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

Wenlan Wang1AEG*, Xiaoming Luo1BD, Qin Zhang1CF, Xujun He2DF, Zhifang Zhang1BF, Xinxin Wang1BC

DOI: 10.12659/MSM.920583

Med Sci Monit 2020; 26:e920583

Abstract

BACKGROUND: Bifidobacteria are among the probiotics used in treating intestinal diseases and are rarely used for allergic asthma treatment. The present study investigated the mechanism of B. infantis in treating allergic asthma in mice.

MATERIAL AND METHODS: A total of 40 male Balb/c mice were randomized into control, ovalbumin (OVA), montelukast (Mon), and B. infantis (B10) groups, and allergic asthma was induced in the OVA, Mon, and B10 groups. Airway reactivity was measured on day 29 by methacholine at various doses. The numbers of total cells and inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted by blood cell counter and Diff-Quik staining. Hematoxylin-eosin (HE) staining was performed to observe inflammatory cell infiltration in lung tissues. Total IgE and OVA-specific IgE in serum were measured by ELISA. Mucin 5AC expression was detected by Western blot to evaluate airway obstruction. The levels of Th1 (IFN-γ, IL-2) and Th2 (IL-4, IL-5, IL-13) cytokines in BALF and tissues were detected by ELISA and qRT-PCR, respectively.

RESULTS: The mice in the OVA group had airway hyperreactivity, while the symptoms in the B10 group and Mon group were effectively relieved. B10 reduced the number of inflammatory cells in BALF as well as inflammatory cell infiltration in tissues. Moreover, the levels of total serum IgE, OVA-specific IgE, and Mucin 5AC were increased in the OVA group, but were reduced in the Mon group and B10 group. B. infantis increased the levels of Th1 cytokines and decreased those of Th2 cytokines.

CONCLUSIONS: B. infantis can reduce the infiltration of inflammatory cells induced by OVA-specific antibodies in mice. B. infantis has therapeutic effects on allergic asthma by promoting Th1 and inhibiting Th2 immune responses.

Keywords: Allergy and Immunology, Asthma, Bifidobacteriales Infections, Ovalbumin, Th1-Th2 Balance, Bifidobacterium longum subspecies infantis, Bronchoalveolar Lavage Fluid, Cytokines, Drugs, Chinese Herbal, Immunoglobulin E, Mice, Inbred BALB C, Random Allocation, Th1 Cells, Th2 Cells

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750