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18 February 2020 : Laboratory Research  

Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis

Ying Hu12ACDE, Feixiang Guo12CE, Hongquan Zhu1ADEFG, Xiaobin Tan3ABCEF, Xiansen Zhu4ABCD, Xiaofeng Liu1ABCDE, Wei Zhang5ABCD, Qiong Yang6ABCD, Yaling Jiang2AG*

DOI: 10.12659/MSM.921502

Med Sci Monit 2020; 26:e921502

Abstract

BACKGROUND: Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer.

MATERIAL AND METHODS: Breast cancer tissue samples were tested using high-throughput sequencing to identify the levels of relative genes; and proteins were addressed by using quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot. Cell ability and cell apoptosis were investigated by Cell Counting Kit-8 (CCK-8) and flow cytometry. Invasion was detected by Transwell invasion assay. The identification of target genes was analyzed by dual-luciferase reporter assay.

RESULTS: Downregulation of circRNA-0001283 expression was observed in breast cancer tissue samples. Ectopic expression of circRNA-0001283 remarkably suppressed cell viability and invasion, and induced apoptosis in breast cancer cells. Furthermore, circRNA-0001283 bound to miR-187 and decreased the expression of miR-187, which resulted in inhibition in cell growth and invasion. Finally, we showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187.

CONCLUSIONS: The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer.

Keywords: DNA, Circular, Neoplasm Invasiveness, Apoptosis, Base Sequence, Breast Neoplasms, Down-Regulation, Gene Expression Regulation, Neoplastic, Intracellular Signaling Peptides and Proteins, MicroRNAs, Protein Serine-Threonine Kinases, RNA, Circular

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750