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17 May 2020 : Laboratory Research  

Knockdown of DANCR Suppressed the Biological Behaviors of Ovarian Cancer Cells Treated with Transforming Growth Factor-β (TGF-β) by Sponging MiR-214

Ping Huang1A*, Bingli Qi1B, Hairong Yao1B, Liang Zhang1C, Yanying Li1C, Qian Li1B

DOI: 10.12659/MSM.922760

Med Sci Monit 2020; 26:e922760

Abstract

BACKGROUND: Ovarian cancer is one of the most common gynecological malignancies and mortality ranks the highest in cancer-associated death in females’ worldwide. Here, we attempted to evaluate the effect of DANCR on the biological behavior of transforming growth factor-β (TGF-β) stimulated ovarian cancer cells.

MATERIAL AND METHODS: The expression of DANCR in ovarian cancer cells (A2780 and SKOV3) treated with TGF-β were detected by quantitative real-time polymerase chain reaction (qRT-PCR). DANCR silencing was constructed using lentiviral transfection in ovarian cancer cells. The Cell Counting Kit-8 (CCK-8), flow cytometry and Transwell assays were performed to measure some cytology index. Western blot was utilized to explore the effect of DANCR on Krüppel-like factor 5 (KLF5) expression.

RESULTS: The expression of DANCR in cancer cells (A2780 and SKOV3) treated with TGF-β was significantly higher. DANCR silencing suppressed cell viability, migration and invasion, and induced cell apoptosis of TGF-β treated ovarian cancer cells. Bioinformatics analysis showed that DANCR served as a sponge for miR-214, and also showed that KLF5 was targeted by miR-214. In addition, DANCR could inhibit the expression of KLF5.

CONCLUSIONS: We are the first to report that knockdown of DANCR could affect the biological process of ovarian cancer cells treated with TGF-β by sponging miR-214, which may provide new therapeutic ideas of ovarian cancer.

Keywords: MicroRNAs, Ovarian Neoplasms, RNA, Long Noncoding, Apoptosis, Transforming Growth Factor beta

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750