14 May 2020 : Clinical Research
miR-20a/Foxj2 Axis Mediates Growth and Metastasis of Colorectal Cancer Cells as Identified by Integrated AnalysisYong Qiang1E, Liang Feng2B, Gang Wang3C, Jian Liu1B, Jing Zhang1C, Lanlan Xiang1D, Chunjie Su1E, Songbai Zhang1F, Xiongwei Xie1F, Erlin Chen4A*
Med Sci Monit 2020; 26:e923559
BACKGROUND: MicroRNAs (miRNAs) have a significant regulatory effect on the proliferation, migration, and invasion of cells, and have been widely reported to have oncogenic or tumor-suppressive impacts on various tumors. In the present study we assessed the regulation and function of miR-20a on colorectal cancer (CRC) cell lines.
MATERIAL AND METHODS: qPCR was used to quantify miR-20a expression. Luciferase reporter assay was conducted to confirm Foxj2 3’UTR associations. In addition, the function of miR-20a and Foxj2 in CRC was detected using MTT, colony formation, transwell assays, and cell cycle analysis.
RESULTS: Our data revealed that miR-20a expression was elevated in the CRC cell lines, and cell migration, proliferation, and invasion abilities were promoted by the overexpression of miR-20a. Moreover, Foxj2 was authenticated as a direct target gene of miR-20a in CRC cells. Furthermore, we found that the ectopic Foxj2 dramatically suppressed miR-20a-promoted proliferation, migration, invasion, and xenografts in vitro and in vivo, and induced cell cycle arrest at G1 stage.
CONCLUSIONS: Our results showing the roles of miR-20a/Foxj2 in carcinogenesis of CRC may help improve treatment of CRC.
Keywords: Colorectal Neoplasms, Hereditary Nonpolyposis, Tumor Markers, Biological, Colorectal Neoplasms, Epithelial-mesenchymal transition, Forkhead Transcription Factors, Gene Expression, Gene Expression Regulation, Neoplastic, Neoplasm Invasiveness, Neoplasm Metastasis
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