Abstract

BACKGROUND: MicroRNAs (miRNAs) have a significant regulatory effect on the proliferation, migration, and invasion of cells, and have been widely reported to have oncogenic or tumor-suppressive impacts on various tumors. In the present study we assessed the regulation and function of miR-20a on colorectal cancer (CRC) cell lines.

MATERIAL AND METHODS: qPCR was used to quantify miR-20a expression. Luciferase reporter assay was conducted to confirm Foxj2 3’UTR associations. In addition, the function of miR-20a and Foxj2 in CRC was detected using MTT, colony formation, transwell assays, and cell cycle analysis.

RESULTS: Our data revealed that miR-20a expression was elevated in the CRC cell lines, and cell migration, proliferation, and invasion abilities were promoted by the overexpression of miR-20a. Moreover, Foxj2 was authenticated as a direct target gene of miR-20a in CRC cells. Furthermore, we found that the ectopic Foxj2 dramatically suppressed miR-20a-promoted proliferation, migration, invasion, and xenografts in vitro and in vivo, and induced cell cycle arrest at G1 stage.

CONCLUSIONS: Our results showing the roles of miR-20a/Foxj2 in carcinogenesis of CRC may help improve treatment of CRC.

Keywords: Colorectal Neoplasms, Hereditary Nonpolyposis, Tumor Markers, Biological, Colorectal Neoplasms, Epithelial-mesenchymal transition, Forkhead Transcription Factors, Gene Expression, Gene Expression Regulation, Neoplastic, Neoplasm Invasiveness, Neoplasm Metastasis