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09 May 2020 : Clinical Research  

CircPSMC3 Suppresses Migration and Invasion of Non-Small Cell Lung Cancer Cells via miR-182-5p/NME2 Axis

Tao Xu1BCDEF, Yejun Yu2BC, Shengcong Guo3DE, Lixiang He3EF, Zejun Mao3AG*

DOI: 10.12659/MSM.924134

Med Sci Monit 2020; 26:e924134

Abstract

BACKGROUND: CircPSMC3 has been reported to play important roles in the occurrence and development of cancer. However, the role of circPSMC3 in NSCLC (non-small cell lung cancer) and the underlying mechanisms remain unclear.

MATERIAL AND METHODS: The expression of circPSMC3 in NSCLC tissues was measured through qRT-PCR (quantitative real-time polymerase chain reaction). The effect of circPSMC3 on the invasion and migration of NSCLC cell line H1299 was determined through transwell invasion assay and wound healing assay. Dual-luciferase reporter assay was performed for exploring the regulatory interaction between circPSMC3, miR-182-5p, and NME2.

RESULTS: Compared with adjacent normal tissues, the expression of circPSMC3 in NSCLC tissues was decreased. Lower circPSMC3 expression was closely associated with lymph node metastasis and higher TNM stage in NSCLC patients. Biological function analysis suggested that circPSMC3 inhibits the invasion and migration of H1299 cells through upregulating the expression of NME2. Mechanistically, circPSMC3 sponges miR-182-5p to suppress the invasion and migration of NSCLC cells via upregulating NME2 expression.

CONCLUSIONS: CircPSMC3 inhibits the invasion and migration of NSCLC cells through the miR-182-5p/NME2 signaling pathway.

Keywords: Genes, Tumor Suppressor, MicroRNAs, A549 cells, ATPases Associated with Diverse Cellular Activities, Gene Expression, Gene Expression Regulation, Neoplastic, NM23 Nucleoside Diphosphate Kinases, Neoplasm Invasiveness, Proteasome Endopeptidase Complex, RNA, Circular

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750