30 December 2008
Ethanol inhibited apoptosis-related RNA binding protein, Napor-3 gene expression in the prenatal rat brainNibedita NahaABDEG, Hae Young LeeBCFG, Mohammad Imran NaserC, Tae Ju ParkF, Sung Hoon KimA, Myeong Ok KimAG
Med Sci Monit 2009; 15(1): BR6-12 :: ID: 869518
Cell death and differentiations are the critical processes in developing fetal brain, where ethanol induces lots of changes in gene expression patterns of fetal nervous system leading to fetal alcohol syndrome (FAS). The objective of the present study was to observe whether maternal ethanol exposure can alter gene expression pattern in mother and in fetus during mid and late prenatal stage.
Material and Method
10% ethanol was orally applied to female Spraque-Dawley rats and fetuses were sacrificed on gestational day (GD) 19.5 and 21.5. Total mRNA was isolated for differential-display PCR (DD-PCR) and sequence was analyzed to find out the homologous gene(s) using GenBank database of the BLAST program. Finally, the gene expression pattern in different maternal and fetal brain areas of the control and the ethanol treated groups were studied by RNase protection assay (RPA) and in situ hybridization.
Out of several differentially expressed genes, apoptosis-related RNA binding protein (RBP), 'Napor-3' mRNA expression was significantly inhibited by ethanol in fetal rat fore-, mid- and hind- brain, and adult rat cortex and hippocampus when compared with the untreated control. The cDNA analysis was further supported our result (accession: AF090697, 95% sequence homology).
The age and area dependent suppression of apoptosis-related RBP, Napor-3 gene expression in proliferating fetal brain by maternal ethanol suggesting high susceptibility towards ethanol intake at the time of neuronal cell development and proliferation. Further, ethanol also affects maternal brain tissues that may be one of the reasons for ethanol-induce irreversible damage of the developing brain. The present study for the first time provides the evidence that Napor-3 suppression by ethanol during mid and late stage fetal brain converts natural physiological event apoptosis into pathological process, which may be useful as a novel therapeutic approach towards FAS-associated developmental brain damage as a consequence of maternal drinking behavior.
Keywords: RNA-Binding Proteins - metabolism, Sequence Analysis, DNA, Nerve Tissue Proteins - metabolism, Molecular Sequence Data, Maternal Exposure - adverse effects, In Situ Hybridization, Gene Expression Regulation, Developmental - drug effects, Ethanol - toxicity, DNA, Complementary - genetics, Brain - metabolism, Base Sequence, Analysis of Variance
01 May 2023 : EditorialEditorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy
Med Sci Monit 2023; 29:e940911
28 May 2023 : Database AnalysisScientometric and Visualized Analysis of Intravoxel Incoherent Motion Magnetic Resonance Imaging: 1988-2021
Med Sci Monit In Press; DOI: 10.12659/MSM.938715
25 May 2023 : Database AnalysisThe COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland
Med Sci Monit In Press; DOI: 10.12659/MSM.940904
25 May 2023 : Review articleA Review of Preclinical and Clinical Studies in Support of the Role of Non-Steroidal Anti-Inflammatory Drug...
Med Sci Monit In Press; DOI: 10.12659/MSM.940635
25 May 2023 : Clinical ResearchPeriapical Lesions and Missed Canals in Endodontically Treated Teeth: A Cone-Beam Computed Tomographic Stud...
Med Sci Monit In Press; DOI: 10.12659/MSM.940533
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952