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25 August 2017 : Laboratory Research  

Cerebrovascular Protective Effect of Boldine Against Neural Apoptosis via Inhibition of Mitochondrial Bax Translocation and Cytochrome C Release

Xiaozhong Qiu1B, Ling Shi1C, Hanting Zhuang1D, Hongtao Zhang1E, Juan Wang2EF, Lijun Wang1DF, Peng Sun1AG, Lili Yu1ABCDEF*, Longxi Liu1AF

DOI: 10.12659/MSM.903040

Med Sci Monit 2017; 23:4109-4116

Abstract

BACKGROUND: In the present study, we explored the protective effect and mechanism of action of boldine (BOL) against neural apoptosis, which is a mediator of TBI.

MATERIAL AND METHODS: The effect of BOL on mitochondrial and cytosol proteins of extracted from cerebral cortical tissue of mice was evaluated. The grip test was used to assess the neurological deficit and brain water content of the subjects after administration of BOL to assess its effect on SOD, GSH, and MDA activity in brain ischemic tissues. To further confirm the effect of the BOL, the histopathological analysis and morphology of neurons were studied by Nissl staining. The effect of BOL against TBI-induced neural apoptosis by immuno-histochemistry and Western blotting assay were also studied.

RESULTS: BOL showed significant improvement against TBI in a dose-dependent manner. In the BOL-treated group, the apoptotic index was significantly reduced, but the level of caspase-3 was greatly diminished. Additionally, the level of the Bax in mitochondria (mit) and cytosol was elevated in the TBI-treated group as compared to the sham group. Further BOL at the test dose causes significant reduction in the level of mitochondrial MDA together with increase in SOD activity as compared to the TBI alone group.

CONCLUSIONS: BOL showed a cerebroprotective effect against TBI by attenuating the oxidative stress and the mitochondrial apoptotic pathway. It also inhibited mitochondrial Bax translocation and cytochrome c release.

Keywords: Cytochromes c, Malondialdehyde

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750