25 August 2017 : Laboratory Research
Cerebrovascular Protective Effect of Boldine Against Neural Apoptosis via Inhibition of Mitochondrial Bax Translocation and Cytochrome C ReleaseXiaozhong Qiu1B, Ling Shi1C, Hanting Zhuang1D, Hongtao Zhang1E, Juan Wang2EF, Lijun Wang1DF, Peng Sun1AG, Lili Yu1ABCDEF*, Longxi Liu1AF
Med Sci Monit 2017; 23:4109-4116
BACKGROUND: In the present study, we explored the protective effect and mechanism of action of boldine (BOL) against neural apoptosis, which is a mediator of TBI.
MATERIAL AND METHODS: The effect of BOL on mitochondrial and cytosol proteins of extracted from cerebral cortical tissue of mice was evaluated. The grip test was used to assess the neurological deficit and brain water content of the subjects after administration of BOL to assess its effect on SOD, GSH, and MDA activity in brain ischemic tissues. To further confirm the effect of the BOL, the histopathological analysis and morphology of neurons were studied by Nissl staining. The effect of BOL against TBI-induced neural apoptosis by immuno-histochemistry and Western blotting assay were also studied.
RESULTS: BOL showed significant improvement against TBI in a dose-dependent manner. In the BOL-treated group, the apoptotic index was significantly reduced, but the level of caspase-3 was greatly diminished. Additionally, the level of the Bax in mitochondria (mit) and cytosol was elevated in the TBI-treated group as compared to the sham group. Further BOL at the test dose causes significant reduction in the level of mitochondrial MDA together with increase in SOD activity as compared to the TBI alone group.
CONCLUSIONS: BOL showed a cerebroprotective effect against TBI by attenuating the oxidative stress and the mitochondrial apoptotic pathway. It also inhibited mitochondrial Bax translocation and cytochrome c release.
Keywords: Cytochromes c, Malondialdehyde
01 February 2024 : EditorialEditorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise
Med Sci Monit 2024; 30:e943911
01 Feb 2024 : Review articleReview of the Evaluation of Pulmonary Hypoplasia as an Important Determinant of Clinical Outcomes in Infant...
Med Sci Monit In Press; DOI: 10.12659/MSM.943259
19 Feb 2024 : Clinical ResearchDiagnostic Accuracy of Ileocolic Artery and Vein Diameter for Acute Appendicitis
Med Sci Monit In Press; DOI: 10.12659/MSM.943846
23 Feb 2024 : Clinical ResearchEarly Mobilization in Pediatric Critical Care: Exploring the Gap Between Theory and Practice in Saudi Arabia
Med Sci Monit In Press; DOI: 10.12659/MSM.942467
Most Viewed Current Articles
16 May 2023 : Clinical ResearchElectrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review articleVaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical ResearchPrevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels
Med Sci Monit 2022; 28:e937990