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30 May 2017 : Laboratory Research  

Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases

Qincai Dong1ABCE, Chenggong Li2BC, Xiuhua Qu3BC, Cheng Cao1AFG, Xuan Liu4AEG*

DOI: 10.12659/MSM.904888

Med Sci Monit 2017; 23:2625-2635

Abstract

BACKGROUND: Studies have found that c-Abl oncogenic kinases may regulate gene transcription by RNA polymerase II phosphorylation or by direct regulation of specific transcription factors or coactivators. However, the global regulation of differential gene expression by c-Abl/Arg is largely unknown. In this study, differentially expressed genes (DEGs) regulated by c-Abl/Arg were identified, and related cellular functions and associated pathways were investigated.

MATERIAL AND METHODS: RNA obtained from wild-type and c-Abl/Arg gene-silenced MCF-7 cells was analyzed by RNA-Seq. DEGs were identified using edgeR software and partially validated by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to explore the potential functions of these DEGs.

RESULTS: A total of 1,034 DEGs were significantly regulated by c-Abl/Arg (399 were up-regulated and 635 were down-regulated after c-Abl/Arg double knockdown). GO and KEGG analyses showed that the DEGs were primarily involved in cellular metabolic processes, neurodegenerative disease, the metabolic process and signaling pathway of cAMP, angiogenesis, and cell proliferation.

CONCLUSIONS: Our data collectively support the hypothesis that c-Abl/Arg regulate differential gene expression, providing new insights into the biological functions of c-Abl and Arg.

Keywords: Proto-Oncogene Proteins c-abl, Sequence Analysis, RNA, Transcription, Genetic

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750