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26 August 2019 : Clinical Research  

Association of Platelet Membrane Fatty Acid Composition with Markers of Oxidative Stress in Healthy Men

Inga Bikulčienė1ABCDEF*, Orinta Golubevaitė1BCDF, Vytautas Žėkas1AB, Mantas Radzevičius1B, Dovilė Karčiauskaitė1ABCDEFG, Rėda Matuzevičienė1ABCD, Vaiva Hendrixson1DE, Asta Mažeikienė1B, Neringa Burokienė2B, Arvydas Kaminskas1ACD, Zita Aušrelė Kučinskienė1ADG

DOI: 10.12659/MSM.915111

Med Sci Monit 2019; 25:6405-6416

Abstract

BACKGROUND: Platelet membranes are extremely susceptible to peroxidation, forming a variety of lipid peroxides, including malondialdehyde (MDA), which has been implicated in the etiology of cardiovascular diseases. Moreover, platelet-leukocyte aggregates (PLAs) are known to contribute to advanced endothelial injury and atherogenesis.

MATERIAL AND METHODS: Fatty acid (FA) methyl esters of the platelet membranes of 79 apparently healthy men without any acute clinical condition at the time of the study were identified by GC/MS. MDA was measured by HPLC in blood serum, and PLAs were analyzed by whole-blood flow cytometry. Individuals were divided into quartiles according to MDA concentration and percentage of PLAs formation. The composition of platelet membrane FAs was compared to MDA concentration and the percentage of PLAs formation in apparently healthy individuals.

RESULTS: In quartiles (Q) with higher MDA concentration, percentage of C 16: 1ω7 (Q₁ vs. Q₃, p=0.021), C 20: 1ω9 (Q₂ vs. Q₄, p=0.028) and C 20: 5ω3 (Q₂ vs. Q₄, p=0.046) was lower. However, C 22: 5ω3 (Q₁ vs. Q₄, p=0.038) and total ω3 (Q₁ vs. Q₂, p=0.024) were higher.

CONCLUSIONS: MDA and the formation of platelet–monocyte aggregates stimulate the incorporation of monounsaturated fatty acids and polyunsaturated fatty acids in platelet phospholipid membranes, which may be a hallmark for a changed level of biologically active compounds required for the activation of future platelets.

Keywords: Malondialdehyde, Platelet Activation, Blood Platelets, Fatty Acids, Fatty Acids, Monounsaturated, Fatty Acids, Unsaturated, Gas Chromatography-Mass Spectrometry, healthy volunteers, Lipid Peroxidation, Phospholipids, Platelet Aggregation

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750