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28 May 2020 : Laboratory Research  

Microfibril-Associated Protein 2 (MFAP2) Potentiates Invasion and Migration of Melanoma by EMT and Wnt/β-Catenin Pathway

Zenghong Chen1ABCDEF*, Yang Lv1ABCDEF, Dongsheng Cao1BCD, Xiaocan Li1BCD, Yuanyi Li1BCD

DOI: 10.12659/MSM.923808

Med Sci Monit 2020; 26:e923808


BACKGROUND: Growing evidence indicates an association between microfibril-associated protein 2 (MFAP2) and a number of physiological and pathological mechanisms. The potential role of MFAP2 in cancer requires further elucidation. The present study investigated the biological behavior of MFAP2 in melanoma patients.

MATERIAL AND METHODS: MFAP2 inhibition was established in the B16 melanoma cell line through the use of RNA interference and was assessed by quantitative real-time PCR (qRT-PCR) and Western blot analysis. Wound-healing analysis, transwell assay, and in vivo imaging were performed to investigate the roles of MFAP2 reducing cell mobility, migration, and invasion abilities in vitro and in vivo.

RESULTS: We found substantially higher MFAP2 expression in B16 melanoma cells. The knockdown of MFAP2 inhibited B16 melanoma cells migration and invasion. Western blot analysis was used to assess changes in biomarkers of EMT, indicating the function of MFAP2 in EMT. We found that downregulation of MFAP2 altered the expression of Wnt/β-catenin-linked protein.

CONCLUSIONS: Our results suggest that MFAP2 has potential as a molecular target to treat melanoma and suppress metastasis of melanoma cells.

Keywords: Epithelial-mesenchymal transition, Melanoma, Amelanotic, Neoplasm Invasiveness, Transcellular Cell Migration, Melanoma, Experimental, Mice, Inbred BALB C, Microfibrils, RNA Interference, RNA Splicing Factors, RNA, Small Interfering, Wnt Signaling Pathway

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01 November 2023 : Editorial  

Editorial: Factors Driving New Variants of SARS-CoV-2, Immune Escape, and Resistance to Antiviral Treatments as the End of the COVID-19 Pandemic is Declared

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville,

DOI: 10.12659/MSM.942960

Med Sci Monit 2023; 29:e942960


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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750