Abstract

BACKGROUND: Growing evidence indicates an association between microfibril-associated protein 2 (MFAP2) and a number of physiological and pathological mechanisms. The potential role of MFAP2 in cancer requires further elucidation. The present study investigated the biological behavior of MFAP2 in melanoma patients.

MATERIAL AND METHODS: MFAP2 inhibition was established in the B16 melanoma cell line through the use of RNA interference and was assessed by quantitative real-time PCR (qRT-PCR) and Western blot analysis. Wound-healing analysis, transwell assay, and in vivo imaging were performed to investigate the roles of MFAP2 reducing cell mobility, migration, and invasion abilities in vitro and in vivo.

RESULTS: We found substantially higher MFAP2 expression in B16 melanoma cells. The knockdown of MFAP2 inhibited B16 melanoma cells migration and invasion. Western blot analysis was used to assess changes in biomarkers of EMT, indicating the function of MFAP2 in EMT. We found that downregulation of MFAP2 altered the expression of Wnt/β-catenin-linked protein.

CONCLUSIONS: Our results suggest that MFAP2 has potential as a molecular target to treat melanoma and suppress metastasis of melanoma cells.

Keywords: Epithelial-mesenchymal transition, Melanoma, Amelanotic, Neoplasm Invasiveness, Transcellular Cell Migration, Melanoma, Experimental, Mice, Inbred BALB C, Microfibrils, RNA Interference, RNA Splicing Factors, RNA, Small Interfering, Wnt Signaling Pathway