Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

22 May 2003

Protein kinase A expression and its possible roles in regulating tooth eruption genes in the dental follicle

Shaomian Yao, Gary E. Wise

Med Sci Monit 2003; 9(5): BR160-167 :: ID: 11044

Abstract

Background: Tooth eruption requires the chronological expression of a series of genes in the dental follicle (DF). Protein kinase A (PKA) is a major phosphorylation pathway in the cells, and may regulate
the expression of tooth eruption genes.
Material/Methods: In vivo, we studied the expression of the regulatory (R) and catalytic (C) subunits of PKA in the DF of newborn rats. In vitro, dental follicle cells (DFC) were treated with a specific PKA
inhibitor, and then the gene expression of monocyte chemotactic protein-1 (MCP-1), colonystimulating factor-1 (CSF-1) and parathyroid hormone-related protein receptor (PTHrP-R) was determined. Cells also were treated with either phorbol-12-myristate-13-acetate or dibutyryl- cAMP, and the gene expression for RIα, RIβ, RIIα and RIIβ of PKA was examined.
Results: The results indicate that RIα of PKA is the predominant subunit in the DF with steady expression from days 1 to 11 postnatally. In contrast, expression of the RIβ, RIIα, RIIβ subunits
are progressively reduced over this time period. However, there is a sharp decline of RIβ expression at postnatal day 3. The expression of the C subunits slightly decreases at days 3 and 5 with a greater decrease at day 7 postnatally. The specific PKA inhibitor reduces MCP-
1 gene expression and translation, as well as moderately reducing CSF-1 and PTHrP-R expression.
Conclusions: The reduction of the RIβ subunit in the rat DF at day 3 may result in an elevated PKA activity to trigger the maximal burst of gene expression of MCP-1 and CSF-1 seen at this time.

Keywords: Animals, Newborn, Chemokine CCL2 - genetics, Chemokine CCL2 - metabolism, Cyclic AMP-Dependent Protein Kinases - chemistry, Cyclic AMP-Dependent Protein Kinases - genetics, Cyclic AMP-Dependent Protein Kinases - metabolism, Dental Sac - growth & development, Dental Sac - metabolism, Gene Expression Regulation, Developmental, Macrophage Colony-Stimulating Factor - genetics, Macrophage Colony-Stimulating Factor - metabolism, Protein Subunits, Receptor, Parathyroid Hormone, Type 1 - genetics, Receptor, Parathyroid Hormone, Type 1 - metabolism, Tooth Eruption - genetics

Add Comment 0 Comments

Editorial

01 May 2024 : Editorial  

Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)

Dinah V. Parums

DOI: 10.12659/MSM.944927

Med Sci Monit 2024; 30:e944927

In Press

Database Analysis  

Long-Term Prognosis of Patients Undergoing Percutaneous Coronary Intervention: The Impact of Serum Creatini...

Med Sci Monit In Press; DOI: 10.12659/MSM.943063  

Database Analysis  

Maternal Exposure to Environmental Air Pollution and Premature Rupture of Membranes: Evidence from Southern...

Med Sci Monit In Press; DOI: 10.12659/MSM.943601  

Animal Research  

Enhanced Bone Healing Through Systemic Capsaicin Administration: An Experimental Study on Wistar Rats

Med Sci Monit In Press; DOI: 10.12659/MSM.942485  

Clinical Research  

Differential Inflammatory Responses in Adult and Pediatric COVID-19 Patients: Implications for Long-Term Co...

Med Sci Monit In Press; DOI: 10.12659/MSM.944052  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750