01 February 2004
Catalytic nucleotide-hydrolyzing antibodies in milk and serum of clinically healthy human mothers
Dmitry V. Semenov, Tatyana G. Kanyshkova, Nataliya A. Karotaeva, Mikhail A. Krasnorutskii, Irina A. Kuznetsova, Valentina N. Buneva, Gregory A. NevinskyMed Sci Monit 2004; 10(2): BR23-33 :: ID: 11575
Abstract
Background:In humans, pregnancy and lactation are associated with the production of catalytically active antibodies (abzymes) in serum and breast milk. However, the substrate specificities of the abzymes in these biological fluids, particularly breast milk, have not been studied extensively.Material/Methods: IgG fractions were isolated from human milk by subsequent steps of chromatographic purification on Protein-A Sepharose, DEAE-cellulose, and anti-IgG Sepharose. The nucleotide-hydrolyzing activity of electrophoretically homogeneous IgG antibodies was measured using 32P-labeled nucleotides and TLC.Results: We demonstrated by different methods that IgG antibodies from the serum and milk of clinically healthy human mothers are able to hydrolyze ribo- and deoxyribonucleoside-5’-mono, di- and triphosphates; this nucleotide-hydrolyzing activity was also present in Fab fragments of the IgG molecule. Affinity modification of the milk IgG oligomeric form by chemically reactive derivatives of ATP led to preferential modification of the L-chain. However, after separation of the subunits by SDS electrophoresis, an in-gel assay showed ATP-hydrolyzing activity in various oligomeric forms of IgG subunits (H2L2, H2L and HL), while the separated heavy (H) and light (L) chains were not catalytically active. The Km and Vmax values characterizing the interaction of IgG with nucleotides were estimated.Conclusions: Our findings speak in favor of the generation of a variety of polyclonal nucleotide-hydrolyzing antibodies by the immune system of clinically healthy mothers.
Keywords: Adenosine Monophosphate - analogs & derivatives, Adenosine Monophosphate - metabolism, Adenosine Triphosphatases - metabolism, Adenosine Triphosphate - analogs & derivatives, Adenosine Triphosphate - metabolism, Antibodies, Catalytic - blood, Antibodies, Catalytic - isolation & purification, Antibodies, Catalytic - metabolism, Hydrolysis, Immunoglobulin Fab Fragments - chemistry, Immunoglobulin Fab Fragments - isolation & purification, Immunoglobulin Fab Fragments - metabolism, Immunoglobulin G - isolation & purification, Immunoglobulin G - metabolism, Immunoglobulin Heavy Chains - metabolism, Immunoglobulin Light Chains - metabolism, Kinetics, Lactation, Milk, Human - chemistry, Milk, Human - immunology, Nucleotides - chemistry, Nucleotides - metabolism, Phosphorus Radioisotopes, Pregnancy, Serum - chemistry, Adenosine Monophosphate - metabolism, Adenosine Triphosphatases - metabolism, Adenosine Triphosphate - metabolism, Antibodies, Catalytic - metabolism, Hydrolysis, Immunoglobulin Fab Fragments - metabolism, Immunoglobulin G - metabolism, Immunoglobulin Heavy Chains - metabolism, Immunoglobulin Light Chains - metabolism, Kinetics, Lactation, Milk, Human - immunology, Nucleotides - metabolism, Phosphorus Radioisotopes, Pregnancy, Serum - chemistry
Editorial
01 November 2024 : Editorial
Editorial: Artificial Intelligence (AI), Digital Image Analysis, and the Future of Cancer Diagnosis and PrognosisDOI: 10.12659/MSM.947038
Med Sci Monit 2024; 30:e947038
In Press
Review article
Comprehensive Insights into Arecoline Hydrobromide: Pharmacology, Toxicity, and PharmacokineticsMed Sci Monit In Press; DOI: 10.12659/MSM.945582
Clinical Research
Iliac Periosteal Bone Autografting vs Talus Non-Weight-Bearing Surgery in Hepple V Talus Osteochondral Inju...Med Sci Monit In Press; DOI: 10.12659/MSM.944912
Clinical Research
Impact of Different Orthodontic Appliances on Mandibular Condyle, Anterior Region of Mandibular, and Maxill...Med Sci Monit In Press; DOI: 10.12659/MSM.946265
Clinical Research
Risk Factors for Bone Cement Displacement After Percutaneous Kyphoplasty in Osteoporotic Vertebral Fracture...Med Sci Monit In Press; DOI: 10.12659/MSM.945884
Most Viewed Current Articles
17 Jan 2024 : Review article 6,371,831
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research 1,909,013
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research 695,468
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
07 Jan 2022 : Meta-Analysis 260,595
Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...DOI :10.12659/MSM.935074
Med Sci Monit 2022; 28:e935074