03 June 2004
Acetylsalicylic acid-dependent inhibition of collagen biosynthesis and ß1 -integrin signaling in cultured fibroblasts
Arkadiusz Surażyński, Jerzy Pałka, Sławomir WołczyńskiMed Sci Monit 2004; 10(6): BR175-179 :: ID: 11675
Abstract
Background:Non-steroid anti-inflammatory drugs (NSAIDs) are known inhibitors of collagen biosynthesis in animal tissues and cell cultures. The mechanism of their action on collagen biosynthesis involves inactivation of prolidase, the enzyme that recovers proline from collagen degradation products for collagen re-synthesis. In fibroblasts, prolidase activity is stimulated through a signal induced by an activated β[sub]1[/sub] -integrin receptor that contributes to an increase in enzyme phosphorylation. To discover whether this is an underlying mechanism of acetylsalicylic acid-dependent inhibition of collagen biosynthesis in fibroblasts was the aim of this study.
Material/Methods: Control and acetylsalicylic acid-treated confluent human skin fibroblasts were compared with respect to collagen biosynthesis ([[sup]3[/sup]H]proline incorporation assay), prolidase activity (colorimetric assay) and the expressions of prolidase and some signaling proteins (Western immunoblot analysis).Results: In cells treated with acetylsalicylic acid, a concomitant decrease in prolidase activity, prolidase phosphorylation (at the threonine residue), and collagen biosynthesis were observed. This was accompanied by a decrease in the expressions of SOS and phosphorylated MAP kinases, ERK1 and ERK2, but not FAK or Grab2. This suggests that a decrease in prolidase activity may contribute to a decrease in the biosynthesis of proline-containing proteins, such as collagen and SOS. A decrease in collagen biosynthesis decreases the interaction of the protein with the β[sub]1[/sub]-integrin receptor (extracellular process), disturbing signaling (intracellular process).Conclusions: The inhibitory effect of acetylsalicylic acid on collagen biosynthesis in fibroblasts is coupled to the inhibition of prolidase phosphorylation (but not expression) and down-regulation of the intracellular signal transmitted by the β[sub]1[/sub] -integrin receptor.
Keywords: Antigens, CD29 - metabolism, Aspirin - pharmacology, Cells, Cultured, Collagen - biosynthesis, Collagen - metabolism, Dipeptidases - metabolism, Fibroblasts, Phosphothreonine - metabolism, Signal Transduction - drug effects, Skin - drug effects, Skin - metabolism, Antigens, CD29 - metabolism, Aspirin - pharmacology, Cells, Cultured, Collagen - metabolism, Dipeptidases - metabolism, Fibroblasts, Phosphothreonine - metabolism, Signal Transduction - drug effects, Skin - metabolism
731 210
Editorial
01 December 2023 : Editorial
Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory InfectionsDOI: 10.12659/MSM.943312
Med Sci Monit 2023; 29:e943312
In Press
01 Dec 2023 : Clinical Research
Risk Factors and Clinical Outcomes of COVID-19 Infection in Multiple Sclerosis Patients: A Retrospective St...Med Sci Monit In Press; DOI:
30 Nov 2023 : Review article
Decoding the Neurological Sequelae of General Anesthesia: A ReviewMed Sci Monit In Press; DOI:
30 Nov 2023 : Clinical Research
Enhanced Pain Relief and Muscle Growth in Individuals with Low Back Instability: The Impact of Blood Flow R...Med Sci Monit In Press; DOI:
29 Nov 2023 : Clinical Research
Comparative Analysis of Intramedullary Nail versus Plate Fixation for Fibula Fracture in Supination Externa...Med Sci Monit In Press; DOI:
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292