Logo Medical Science Monitor

Call: +1.631.470.9640
Closed: National Holiday

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 August 2004

Catalytic properties of IgMs with amylolytic activity isolated from patients with multiple sclerosis

Dina R. Ivanen, Anna A. Kulminskaya, Konstantin A. Shabalin, Luydmila V. Isaeva-Ivanova, Nadezhda A. Ershova, Andrew N. Saveliev, Gregory A. Nevinsky, Kirill N. Neustroev

Med Sci Monit 2004; 10(8): BR273-280 :: ID: 11725

Abstract

Background:Recently, amylolytic activity was detected in IgMs isolated from the sera of the patients with multiple sclerosis.Material/Methods: All purified samples of IgM were electrophoretically homogenous and did not contain any co-purified α-amylase and α-glucosidase activities, in accordance with a set of criteria developed for abzymes. The amylolytic activity of abzymes was studied in the hydrolysis of p-nitrophenyl α-D-maltooligosaccharides with different degrees of polymerization from 1 to 8 by TLC and reverse-phase HPLC techniques.Results: All IgM samples isolated from 54 patients with clinically definite multiple sclerosis demonstrated hydrolytic activity towards the above artificial substrates. The Michaelis constant values (Km) in the hydrolysis of p-nitrophenyl α-D-maltoheptaoside were in the range of 10 p-nitrophenyl or p-nitrophenyl α-D-glucosides, thus indicating the presence of an α-D-glucosidase activity. For a number of the investigated samples, specific amylolytic activity increased depending on the length of substrates (from p-nitrophenyl maltopentaoside to p-nitrophenyl maltohexaoside); for other IgMs, the opposite dependence was observed. All IgMs studied did not exhibit any other glycoside hydrolase activities toward p-nitrophenyl glycoside substrates.Conclusions: Abzyme fractions from different donors demonstrated catalytic heterogeneity in Michaelis-Menten parameters and different modes of action in the hydrolysis of p-nitrophenyl maltooligosaccharides. Enzymatic properties of the IgMs tested varied from human a-amylases. All investigated abzyme samples did not show transglycosylating ability.

Keywords: Nitrobenzenes - chemistry, Amylases - metabolism, Antibodies, Catalytic - blood, Chromatography, High Pressure Liquid, Immunoglobulin M - metabolism, Multiple Sclerosis - enzymology, Nitrobenzenes - chemistry, Oligosaccharides - chemistry

Add Comment 0 Comments

Editorial

01 November 2024 : Editorial  

Editorial: Artificial Intelligence (AI), Digital Image Analysis, and the Future of Cancer Diagnosis and Prognosis

Dinah V. Parums

DOI: 10.12659/MSM.947038

Med Sci Monit 2024; 30:e947038

0:00

In Press

Clinical Research  

Iliac Periosteal Bone Autografting vs Talus Non-Weight-Bearing Surgery in Hepple V Talus Osteochondral Inju...

Med Sci Monit In Press; DOI: 10.12659/MSM.944912  

Clinical Research  

Impact of Different Orthodontic Appliances on Mandibular Condyle, Anterior Region of Mandibular, and Maxill...

Med Sci Monit In Press; DOI: 10.12659/MSM.946265  

Clinical Research  

Risk Factors for Bone Cement Displacement After Percutaneous Kyphoplasty in Osteoporotic Vertebral Fracture...

Med Sci Monit In Press; DOI: 10.12659/MSM.945884  

Clinical Research  

Adipose-Derived Mesenchymal Stem Cells from Arthritis Patients: Differential Modulation of CD4⁺ T Cell Acti...

Med Sci Monit In Press; DOI: 10.12659/MSM.945273  

Most Viewed Current Articles

17 Jan 2024 : Review article   6,407,835

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,909,112

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   695,529

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

07 Jan 2022 : Meta-Analysis   260,637

Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...

DOI :10.12659/MSM.935074

Med Sci Monit 2022; 28:e935074

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750