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15 July 2002

Biophysical characterization of albumin preparations from blood serum of healthy donors and patients with renal diseases. Part I: Spectrofluorometric analysis

Tatiana V. Parkhomenko, Oksana A. Klicenko, Mikhail M. Shavlovski, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin T. Turoverov

Med Sci Monit 2002; 8(7): BR261-265 :: ID: 13615

Abstract

Background: Serum albumin (SA) is a major proteinacous component of the blood plasma. In the present study we report on the determination and structural characterization of the modified SA in
the plasma of glomerulonephritis (GN) patients. Fluorescence analysis of albumin-enriched fractions (AEF) isolated from serum of GN patients leads to the discovery of a proteinacous component with essential blue shift of intrinsic fluorescence.
Material/Methods: AEF were isolated from serum blood of 35 GN patients, 30 GN patients with chronic renal failure (CRF) corrected by hemodialysis (HD) and 40 healthy donors. Their fluorescence characteristics have been compared using variations in the value of parameter A = I320/I365 that characterizes fluorescence spectrum position and shape.
Results: The fluorescence spectra of AEF from GN and GN with CRF patients were blue- and redshifted, respectively, in comparison with spectra of donors’ serum. The value of parameter A was 1.27±0.05 for protein preparations from healthy donors, while this characteristic lies
within range of 1.3–2.1 or 0.77–1.29 for GN and GN with CRF patients, respectively. Moreover, for GN patients, the magnitude of spectral parameter A was approaching control values during the treatment of disease.
Conclusion: hSA enriched fractions isolated from GN patients or GN patients with CRF corrected by HD differ by their spectrofluorometric properties from those of healthy donors. This technique allows the detection of renal diseases and the efficiency of their cure.

Keywords: Glomerulonephritis - blood, Glomerulonephritis - therapy, Kidney Failure, Chronic - blood, Kidney Failure, Chronic - therapy, Serum Albumin - chemistry, Serum Albumin - metabolism, Spectrometry, Fluorescence, Statistics

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750