Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

Poly(ADP-ribosyl)ation, DNA repair and life span

A. Bürkle, S. Beneke, C. Brabeck, A. Leake, R. Meyer, M. L. Muiras, R. Pfeiffer

Med Sci Monit 2003; 9(1): 5-0 :: ID: 14990

Abstract

Poly(ADP-ribosyl)ation is functionally associated with DNA base-excision repair and is a survival factor and negative regulator of genomic instability in cells under low to moderate levels of genotoxic stress [1]. Previously we have described a positive correlation between poly(ADP-ribosyl)ation capacity of mononuclear blood cells with longevity of mammalian species [2]. Our subsequent comparisons of purified recombinant human and rat PARP-1 revealed that this correlation might be explained in part by evolutionary sequence divergence [3]. We have also reported an association of high poly(ADP-ribosyl)ation capacity of lymphoblastoid cell cultures with longevity of human donors [4]. Recently we have asked if the efficiency of single strand-break repair in mononuclear blood cells following gamma-radiation might also be related with mammalian life span. We quantified DNA strand breaks both immediately after irradiation and after a defined postincubation (‘repair’) period, using a recently developed automated version of the fluorescence-detected alkaline unwinding assay. Our data reveal the existence of a highly significant positive correlation between the extent of repair and life span of donor species [5]. In another set of experiments we have investigated the possible effects of L-selegiline on the cellular poly(ADP-ribosyl)ation response. L-selegiline is an anti-Parkinson drug, which irreversibly inhibits monoamine oxidase B (MAO-B) but possesses additional effects, such as neuroprotective activity in vivo and in cultured cells and life span-extension in laboratory animals. Intriguingly, our data show that low concentrations of L-selegiline can potentiate the gamma-radiation-induced poly(ADP-ribose) formation in cultured hamster cells [6], thus strengthening the links between poly(ADP-ribosyl)ation, cytoprotection and mammalian longevity.References: 1.Bürkle A: Cancer Lett, 2001; 163: 1-5 2.Grube K, Bürkle A: Proc Natl Acad Sci USA, 1992; 89: 11759-11763 3.Beneke S, Alvarez-Gonzalez R, Bürkle A: Exp Gerontol, 2000; 35: 989-1002 4.Muiras ML, Müller M, Schächter F, Bürkle A: J Mol Med, 1998; 76: 346-354 5.Pfeiffer R, Leake A, Müller M, Kirkwood TB, Bürkle A: manuscript in preparation. 6.Brabeck C, Pfeiffer R, Leake L, Beneke S, Meyer R, Bürkle A: submitted.

Keywords: PARP-1, DNA strand breaks, DNA Repair, ageing, life span, L-selegiline

Add Comment 0 Comments

Editorial

01 April 2025 : Editorial  

Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to Humans

Dinah V. Parums

DOI: 10.12659/MSM.949109

Med Sci Monit 2025; 31:e949109

0:00

In Press

Clinical Research  

Impact of Voltage Level on Hospitalization and Mortality in Electrical Injury Cases: A Retrospective Analys...

Med Sci Monit In Press; DOI: 10.12659/MSM.947675  

0:00

Clinical Research  

Comparative Study of Plantar Load and Foot Posture Characteristics in Male Elite Squash Players and Non-Ath...

Med Sci Monit In Press; DOI: 10.12659/MSM.947828  

0:00

Clinical Research  

Dental Students’ Opinions on Use of Artificial Intelligence: A Survey Study

Med Sci Monit In Press; DOI: 10.12659/MSM.947658  

0:00

Database Analysis  

Defining Effect Size Standards in Temporomandibular Joint and Masticatory Muscle Research

Med Sci Monit In Press; DOI: 10.12659/MSM.948365  

0:00

Most Viewed Current Articles

17 Jan 2024 : Review article   8,126,196

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

16 May 2023 : Clinical Research   703,007

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Mar 2024 : Editorial   30,250

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...

DOI :10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

28 Jan 2024 : Review article   24,107

A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and Future

DOI :10.12659/MSM.943912

Med Sci Monit 2024; 30:e943912

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750