02 May 2003
Transcriptional coactivation of NF-kB by p300 is mediated by PARP-1
P. Hassa, C. Burki, C. Lombardi, R. Imhof, M. HottigerMed Sci Monit 2003; 9(1): 27-0 :: ID: 15079
Abstract
NF-kB plays an important role in the transcriptional regulation of a variety of genes involved in immune and inflammatory responses and cell survival. There has been increasing experimental evidence suggesting that abnormalities in the regulation of NF-kB activity are tightly linked to the pathogenesis of inflammatory disorders, neurodegenerative diseases as well as chronic inflammatory diseases. The transcriptional activation by NF-kB requires interactions with multiple cofactors. We earlier provided evidence that NF-kB interacts directly with Poly-(ADP-ribose) polymerase-1 (PARP-1) through both subunits of NF-kB p65 and p50, respectively. The enzymatic as well as the DNA binding activity of PARP-1 was not required for NF-kB-dependent gene expression. NF-kB has also been described to interact with the transcriptional coactivator p300. Interestingly, in PARP-1 knock out cells the recruitment of the transcriptional coactivator p300 by NF-kB was not sufficient for NF-kB-dependent gene expression. Functional complementation revealed that the C-terminal end of PARP-1 was required for transcriptional activity of NF-kB in response to TNF-a and LPS, but not PMA. Moreover, we will provide evidence that PARP-1 plays an essential regulatory role in NF-kB-dependent gene expression by enhancing synergistically with p300 the assembly of a transcriptional pre-initiation complex. References: 1.Hassa PO, Hottiger MO: A role of poly (ADP-ribose) polymerase in NF-kB transcriptional activation. Biol Chem, 1999; 380: 953-959 2.Hassa PO, Covic M, Hasan S, Imhof R, Hottiger MO: The Enzymatic and DNA binding Activity of PARP-1 Are Not Required for NF-kB Coactivator Function. J Biol Chem, 2001; 276(49): 45588-45597 3.Hassa PO, Hottiger MO: The Functional Role of Poly(ADP Ribose)Polymerase 1 (PARP-1) in Inflammatory Disorders. Cell Mol Life Sci, 2002; 59: 1534-1553
Keywords: PARP-1, NF-kB, p300, transcriptional coactivator
Editorial
01 April 2025 : Editorial
Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to HumansDOI: 10.12659/MSM.949109
Med Sci Monit 2025; 31:e949109
In Press
Clinical Research
Impact of Depression, Fatigue, and Pain on Quality of Life in Slovak Multiple Sclerosis PatientsMed Sci Monit In Press; DOI: 10.12659/MSM.947630
Clinical Research
Longitudinal Evaluation of Metabolic Benefits of Inactivated COVID-19 Vaccination in Diabetic Patients in T...Med Sci Monit In Press; DOI: 10.12659/MSM.947450
Clinical Research
Impact of Voltage Level on Hospitalization and Mortality in Electrical Injury Cases: A Retrospective Analys...Med Sci Monit In Press; DOI: 10.12659/MSM.947675
Clinical Research
Comparative Study of Plantar Load and Foot Posture Characteristics in Male Elite Squash Players and Non-Ath...Med Sci Monit In Press; DOI: 10.12659/MSM.947828
Most Viewed Current Articles
17 Jan 2024 : Review article 8,044,083
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 702,972
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 30,118
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 24,024
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912