Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

Transcriptional coactivation of NF-kB by p300 is mediated by PARP-1

P. Hassa, C. Burki, C. Lombardi, R. Imhof, M. Hottiger

Med Sci Monit 2003; 9(1): 27- :: ID: 15079

Abstract

NF-kB plays an important role in the transcriptional regulation of a variety of genes involved in immune and inflammatory responses and cell survival. There has been increasing experimental evidence suggesting that abnormalities in the regulation of NF-kB activity are tightly linked to the pathogenesis of inflammatory disorders, neurodegenerative diseases as well as chronic inflammatory diseases. The transcriptional activation by NF-kB requires interactions with multiple cofactors. We earlier provided evidence that NF-kB interacts directly with Poly-(ADP-ribose) polymerase-1 (PARP-1) through both subunits of NF-kB p65 and p50, respectively. The enzymatic as well as the DNA binding activity of PARP-1 was not required for NF-kB-dependent gene expression. NF-kB has also been described to interact with the transcriptional coactivator p300. Interestingly, in PARP-1 knock out cells the recruitment of the transcriptional coactivator p300 by NF-kB was not sufficient for NF-kB-dependent gene expression. Functional complementation revealed that the C-terminal end of PARP-1 was required for transcriptional activity of NF-kB in response to TNF-a and LPS, but not PMA. Moreover, we will provide evidence that PARP-1 plays an essential regulatory role in NF-kB-dependent gene expression by enhancing synergistically with p300 the assembly of a transcriptional pre-initiation complex. References: 1.Hassa PO, Hottiger MO: A role of poly (ADP-ribose) polymerase in NF-kB transcriptional activation. Biol Chem, 1999; 380: 953-959 2.Hassa PO, Covic M, Hasan S, Imhof R, Hottiger MO: The Enzymatic and DNA binding Activity of PARP-1 Are Not Required for NF-kB Coactivator Function. J Biol Chem, 2001; 276(49): 45588-45597 3.Hassa PO, Hottiger MO: The Functional Role of Poly(ADP Ribose)Polymerase 1 (PARP-1) in Inflammatory Disorders. Cell Mol Life Sci, 2002; 59: 1534-1553

Keywords: PARP-1, NF-kB, p300, transcriptional coactivator

Add Comment 0 Comments

282 0

Editorial

01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209

0:00

In Press

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

01 Jun 2023 : Clinical Research  

Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.940873  

01 Jun 2023 : Clinical Research  

Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.940187  

01 Jun 2023 : Clinical Research  

Influence of Insulin Resistance on Diabetes Nephropathy

Med Sci Monit In Press; DOI: 10.12659/MSM.939482  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

0:00

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750