Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

PARP-1 involvement in growth inhibitory effects of temozolomide and topotecan in p53 wild-type (D54) and p53 mutated (U251) glioblastoma cells

S. Pepe, G. Cimmino, D. Melisi, G. Laus, S. Di Meglio, G. Tortora, A. Bianco, P. Quesada

Med Sci Monit 2003; 9(1): 56- :: ID: 15121

Abstract

Malignant gliomas occur more frequently than other types of primary central nervous system tumors. Among the chemotherapic agents, temozolomide (TZM) and topotecan (TPT) represent important new drugs active in such neoplastic diseaseas. Moreover, preclinical studies have evaluated the combination of these agents and inhibitors of the DNA repair enzymes [1].In the present study we investigated cell growth inhibition and cell cycle perturbation induced by TPT or TZM treatment in the presence of the specific PARP-1 inhibitor NU 1025 in two gliobastoma cell lines, the D54 (p53 wt) and the U251 (p53 mut). Agar-colony-forming assay showed a different sensitivity of both cell lines to TPT or TZM. Moreover, combined treatment with TZM and non cytotoxic concentration of NU1025 induced a 25% increase of growth inhibition in D54 and U251; the same additive effect was observed mainly in U251 for TPT plus NU1025 treatment. DNA-flow cytometry showed that TZM and TPT induce an accumulation of cells in G2M phase of cell cycle and that PARP-1 inhibitor addition doubled G2M block in TZM treated cells.The characterization of the poly(ADP-ribosyl)ation system in both glioblastoma cell lines is in progress and particular attention will be made to define PARP-1 involvement in p53 dependent and independent signal transduction system.References: 1.Friedman SH, Kerby T, Calvert H: Clin Cancer Res, 2000; 6: 2585-2597

Keywords: PARP-1, temozolomide, Topotecan, p53, Cells

0 Comments

Editorial

01 March 2023 : Editorial  

Editorial: Global Surveillance of Highly Pathogenic Avian Influenza Viruses in Poultry, Wild Birds, and Mammals to Prevent a Human Influenza Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939968

Med Sci Monit 2023; 29:e939968

SARS-CoV-2/COVID-19

29 March 2023 : Editorial  

Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...

Med Sci Monit In Press; DOI: 10.12659/MSM.940519  

30 March 2023 : Clinical Research  

An Online Survey on the Relationship Between Positive Coping and Post-Traumatic Stress Symptoms (PTSS) of M...

Med Sci Monit In Press; DOI: 10.12659/MSM.939485  

09 February 2023 : Clinical Research  

Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...

Med Sci Monit 2023; 29:e938892

In Press

30 Mar 2023 : Clinical Research  

An Online Survey on the Relationship Between Positive Coping and Post-Traumatic Stress Symptoms (PTSS) of M...

Med Sci Monit In Press; DOI: 10.12659/MSM.939485  

30 Mar 2023 : Database Analysis  

Global Research Trends and Hotspots in Lateral Epicondylitis During the Past 30 Years: A Bibliometric and V...

Med Sci Monit In Press; DOI: 10.12659/MSM.939309  

29 Mar 2023 : Editorial  

Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...

Med Sci Monit In Press; DOI: 10.12659/MSM.940519  

29 Mar 2023 : Laboratory Research  

Preparation, Physicochemical Properties, and Structural Characterization of Resistant Starch-Aspirin-Loaded...

Med Sci Monit In Press; DOI: 10.12659/MSM.939711  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750