Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003 : Case report  

Peroxynitrite-induced toxicity in spinal cord neurons is dependent on poly(ADP-ribose) polymerase activation

G. Scott, D. Hooper, C. Szabo

Med Sci Monit 2003; 9(1): 59-0 :: ID: 15198

Abstract

Spinal cord trauma initiates a series of sequential events that lead to neuronal cell death. While the precise mechanisms responsible for this secondary damage remain undefined, several studies have suggested that free radicals contribute to the neurodestructive processes of spinal cord injury [1]. Moreover, peroxynitrite, a highly reactive molecule generated when nitric oxide and superoxide combine, has recently been proposed to induce secondary neuronal damage resulting from spinal cord injury [2–5]. Peroxynitrite can mediate several destructive interactions, including activation of poly(ADP-ribose) polymerase (PARP). In the present study we have examined whether peroxynitrite exerts toxic effects on primary cultures of spinal cord neurons via activation of PARP. Peroxynitrite dose-dependently reduced the viability of spinal cord neurons in culture and induced cell death. Furthermore, exposure to peroxynitrite markedly increased DNA strand breaks in spinal cord neurons resulting in activation of PARP. To determine whether PARP activation plays a role in peroxynitrite-induced neurotoxicity we assessed the effects of various PARP inhibitors on cell viability and death in spinal cord neurons exposed to peroxynitrite. The presence of PARP inhibitors in the cultures protected spinal cord neurons from the deleterious actions of peroxynitrite. Therefore, our results demonstrate that peroxynitrite exerts cytotoxic effects on spinal cord neurons in vitro through a PARP-dependent pathway. References: 1.Anderson DK, Hall ED: Pathophysiology of spinal cord trauma. Ann Emerg Med, 1993; 22: 987-992 2.Scott GS, Jakeman LB, Stokes BT, Szabó C: Peroxynitrite production and activation of poly(adenosine diphosphate-ribose) synthetase in spinal cord injury. Ann Neurol, 1999; 45: 120-124 3.Liu D, Ling X, Wen J, Liu J: The role of reactive nitrogen species in secondary spinal cord injury: Formation of nitric oxide, peroxynitrite and nitrated protein. J Neurochem, 2000; 75: 2144-2154 4.Xu J, Kim G-M, Chen S, Yan P et al: iNOS and nitrotyrosine expression after spinal cord injury. J Neurotrauma, 2001; 18: 523-532 5.Bao F, Liu D: Peroxynitrite generated in the rat spinal cord induces neuron death and neurological deficits. Neuroscience, 2002; 115: 839-849

Keywords: spinal cord injury, peroxynitrite, PARP

Add Comment 0 Comments

Editorial

01 June 2024 : Editorial  

Editorial: Concerns as Highly Pathogenic Avian Influenza (HPAI) Virus of the H5N1 Subtype is Identified in Dairy Cows and Other Mammals

Dinah V. Parums

DOI: 10.12659/MSM.945315

Med Sci Monit 2024; 30:e945315

0:00

In Press

Clinical Research  

Functional Outcomes of Therapeutic Selective Nerve Root Block for Single-Segment Lumbar Spinal Stenosis: A ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943634  

Clinical Research  

Shortening Oral Simethicone-to-Colonoscopy Interval Increases Bowel Preparation Quality

Med Sci Monit In Press; DOI: 10.12659/MSM.943972  

Clinical Research  

Comparative Evaluation of Digital Cephalometric Tracing Applications on Mobile Devices and Manual Tracing

Med Sci Monit In Press; DOI: 10.12659/MSM.944628  

Clinical Research  

Impact of COVID-19-Induced Academic Stress on Insomnia and Suicidal Ideation among Taiwanese Health Trainee...

Med Sci Monit In Press; DOI: 10.12659/MSM.944932  

Most Viewed Current Articles

17 Jan 2024 : Review article   1,801,647

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,532,330

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   690,353

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial   50,289

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750