Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

05 May 2005 : Original article  

Regulation of various genes in human leukocytes acutely exposed to morphine: expression microarray analysis

George B. Stefano, John D. Burrill, Sergei Labur, Julie Blake, Patrick Cadet

Med Sci Monit 2005; 11(5): MS35-42 :: ID: 16159

Abstract

Background:Previous studies from our laboratory have identified a novel mu opiate receptor µ[sub]3[/sub], which is expressed in several different cell types and tissues including human vascular endothelial cells, leukocytes and neural tissues. This novel µ receptor is selective for the opiate alkaloid morphine, since this receptor does not bind other opioid peptides.Material/Methods:This report details the acute affects of morphine exposure (1 µg/ml) to human leukocytes by analyzing gene expression using microarrays (Applied Biosystems). Robust estimation of the median fold change was used to identify candidates for significantly differentially expressed genes. An independent experiment using four same sample arrays was used to test the algorithm and to confirm the calculated percentage of falsely significant genes.Results:Data obtained from this study demonstrate that acute morphine exposure differentially affected genes that are involved in immune function, signal transduction, cell adhesion, and apoptosis.Conclusions:Acute morphine exposure to human leukocytes results in specific and significant alterations in gene expression.

Keywords: Gene Expression Profiling - statistics & numerical data, Receptors, Opioid, mu - metabolism, Algorithms, Gene Expression Profiling - statistics & numerical data, Gene Expression Regulation - drug effects, Interleukin-2 - genetics, Leukocytes - metabolism, Morphine - pharmacology, Oligonucleotide Array Sequence Analysis - statistics & numerical data, Receptors, Opioid, mu - metabolism, Signal Transduction - genetics, Tumor Necrosis Factor-alpha - genetics

Add Comment 0 Comments

Editorial

01 April 2025 : Editorial  

Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to Humans

Dinah V. Parums

DOI: 10.12659/MSM.949109

Med Sci Monit 2025; 31:e949109

0:00

In Press

Clinical Research  

Use of The Mirror-Image Technique for Patient-Specific Reconstruction of Cuboid Fractures

Med Sci Monit In Press; DOI: 10.12659/MSM.947437  

Laboratory Research  

Retention of 3-D-Printed, Milled, and Conventionally Fabricated Occlusal Splints

Med Sci Monit In Press; DOI: 10.12659/MSM.947342  

Review article  

A Review of Circulating Tumor DNA (ctDNA) and the Liquid Biopsy in Cancer Diagnosis, Screening, and Monitor...

Med Sci Monit In Press; DOI: 10.12659/MSM.949300  

Clinical Research  

Buccal Acupuncture Reduces the Dose of Sufentanil Needed in Laparoscopic Gynecological Surgery

Med Sci Monit In Press; DOI: 10.12659/MSM.947088  

Most Viewed Current Articles

17 Jan 2024 : Review article   7,932,846

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

16 May 2023 : Clinical Research   702,905

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Mar 2024 : Editorial   29,808

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...

DOI :10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

28 Jan 2024 : Review article   23,790

A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and Future

DOI :10.12659/MSM.943912

Med Sci Monit 2024; 30:e943912

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750