05 May 2005 : Original article
Regulation of various genes in human leukocytes acutely exposed to morphine: expression microarray analysis
George B. Stefano, John D. Burrill, Sergei Labur, Julie Blake, Patrick CadetMed Sci Monit 2005; 11(5): MS35-42 :: ID: 16159
Abstract
Background:Previous studies from our laboratory have identified a novel mu opiate receptor µ[sub]3[/sub], which is expressed in several different cell types and tissues including human vascular endothelial cells, leukocytes and neural tissues. This novel µ receptor is selective for the opiate alkaloid morphine, since this receptor does not bind other opioid peptides.Material/Methods:This report details the acute affects of morphine exposure (1 µg/ml) to human leukocytes by analyzing gene expression using microarrays (Applied Biosystems). Robust estimation of the median fold change was used to identify candidates for significantly differentially expressed genes. An independent experiment using four same sample arrays was used to test the algorithm and to confirm the calculated percentage of falsely significant genes.Results:Data obtained from this study demonstrate that acute morphine exposure differentially affected genes that are involved in immune function, signal transduction, cell adhesion, and apoptosis.Conclusions:Acute morphine exposure to human leukocytes results in specific and significant alterations in gene expression.
Keywords: Gene Expression Profiling - statistics & numerical data, Receptors, Opioid, mu - metabolism, Algorithms, Gene Expression Profiling - statistics & numerical data, Gene Expression Regulation - drug effects, Interleukin-2 - genetics, Leukocytes - metabolism, Morphine - pharmacology, Oligonucleotide Array Sequence Analysis - statistics & numerical data, Receptors, Opioid, mu - metabolism, Signal Transduction - genetics, Tumor Necrosis Factor-alpha - genetics
Editorial
01 April 2025 : Editorial
Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to HumansDOI: 10.12659/MSM.949109
Med Sci Monit 2025; 31:e949109
In Press
Clinical Research
Use of The Mirror-Image Technique for Patient-Specific Reconstruction of Cuboid FracturesMed Sci Monit In Press; DOI: 10.12659/MSM.947437
Laboratory Research
Retention of 3-D-Printed, Milled, and Conventionally Fabricated Occlusal SplintsMed Sci Monit In Press; DOI: 10.12659/MSM.947342
Review article
A Review of Circulating Tumor DNA (ctDNA) and the Liquid Biopsy in Cancer Diagnosis, Screening, and Monitor...Med Sci Monit In Press; DOI: 10.12659/MSM.949300
Clinical Research
Buccal Acupuncture Reduces the Dose of Sufentanil Needed in Laparoscopic Gynecological SurgeryMed Sci Monit In Press; DOI: 10.12659/MSM.947088
Most Viewed Current Articles
17 Jan 2024 : Review article 7,932,846
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 702,905
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 29,808
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 23,790
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912