Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 July 2005 : Original article  

Placental Leucine Aminopeptidase Might Regulate the Effects of Oxytocin with Resolution in Endothelial Cells.

Michiyo Sano, Atsuo Itakura, Mitsuaki Ito, Mikihito Takeuchi, Mayumi Okada, Tomomi Kotani, Shigehiko Mizutani, Fumitaka Kikkawa

Med Sci Monit 2005; 11(7): BR195-199 :: ID: 16970

Abstract

Background: Oxytocin (OT) is known to cause vascular relaxation via nitricoxide (NO) production and proliferation of endothelial cells. Previously we revealed that human umbilicalvascular endothelial cells (HUVECs) expresses placental leucine aminopeptidase (P-LAP)/insulin regulatedaminopeptidase (IRAP), which becomes translocation to the cell surface on activation of oxytocin receptor(OTR). However, the physiological roles of P-LAP in HUVECs have not been elucidated. Material/Methods:Actions of OT on HUVECs transfected with a copy of the human P-LAP gene were therefore examined witha focus on changes in parameters linked to OTR such as calcium mobilization, NO production and cell proliferation.Results: Cell surface P-LAP activity was significantly elevated (~3 fold) in P-LAP overexpressing-HUVECsand overexpression of P-LAP resulted in remarkable inhibition of OT effects on HUVECs such as cell proliferation,[Ca(2+)]i, and NO production. Conclusions: These findings suggested that P-LAP on the plasma membranein HUVECs regulates the effects of OT with resolution around OTR.

Keywords: Cystinyl Aminopeptidase - biosynthesis, Cystinyl Aminopeptidase - genetics, Calcium - metabolism, Cell Proliferation - drug effects, Cystinyl Aminopeptidase - genetics, Endothelial Cells - metabolism, Endothelium, Vascular - metabolism, Nitric Oxide - biosynthesis, Oxytocin - pharmacology, Umbilical Veins - cytology, Vasodilator Agents - pharmacology

Add Comment 0 Comments

Editorial

01 July 2024 : Editorial  

Editorial: The Global Threats of Increasing Antimicrobial Resistance Require New Approaches to Drug Development, Including Molecular Antimicrobial Adjuvants

Dinah V. Parums

DOI: 10.12659/MSM.945583

Med Sci Monit 2024; 30:e945583

0:00

In Press

Database Analysis  

Local Government Initiatives: A Comprehensive Analysis of Infertility Diagnostic and Treatment Programs in ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943976  

0:00

Clinical Research  

Gender-Specific Risk Factors for Asymmetric Bone Density in Adult Degenerative Lumbar Scoliosis: A Retrospe...

Med Sci Monit In Press; DOI: 10.12659/MSM.944137  

Review article  

Innovative Approaches to Enhance and Measure Medication Adherence in Chronic Disease Management: A Review

Med Sci Monit In Press; DOI: 10.12659/MSM.944605  

Clinical Research  

Effects of Ozone Disc Nucleolysis in Management of Herniated Lumbar Intervertebral Disc: A Retrospective Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.944645  

Most Viewed Current Articles

17 Jan 2024 : Review article   3,694,697

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,585,228

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   690,866

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial   50,699

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750