Logo Medical Science Monitor

Call: +1.631.470.9640
Closed: National Holiday

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 August 2005

NO(2)-induced acute and chronic lung injury cause imbalance of glutathionemetabolism in type II pneumocytes.

Renate Hochscheid, Ute Schuchmann, Elke Kotte, Stephan Kranz, Sven Heinrichs, Bernd Muller

Med Sci Monit 2005; 11(8): BR273-279 :: ID: 200945

Abstract

Background: During inspiration the lung is exposed to numerous oxidantsand therefore has developed a system of antioxidant defense. This organ, besides the liver, is the majorsource of glutathione (GSH) metabolism, from which type II pneumocytes are metabolically the most activecells. Material/Methods: To analyze oxidative stress, rats were exposed to air (control) or to 10 ppmnitrogen dioxide (NO(2)) for 3 and 20 days to induce acute and chronic lung injury. As measure of oxidativestress, GSH/GSSG ratios in blood, bronchoalveolar lavage (BAL) and type II pneumocytes were determined.Lipid peroxidation (LPO) was also measured in type II cells. To investigate the basis of these observations,GSH metabolism in type II pneumocytes was studied, analyzing mRNA expression of g-glutamylcystein synthetase(gamma-GCS), glutathione synthetase (GS), gamma-glutamyltranspeptidase (gamma-GT), glutathione peroxidases(GPXs) and glutathione reductase (GR). Furthermore, enzyme activities of GPX and GR were determined.Results: In acute and chronic lung injury the GSH/GSSG ratio was reduced in blood and BAL, but therewas no change in type II pneumocytes. LPO in type II cells was only reduced in acute lung injury. Inboth kinds of lung injury mRNA expression of gamma-GCS, GS and GPX3 decreased, while expression of gamma-GTand GR increased. GPX4 mRNA expression decreased in acute lung injury and increased in the chronic state.Enzyme activity of GPX and GR was generally increased in lung injury. Conclusions: In NO(2) induced acuteand chronic lung injury, GSH metabolism is imbalanced.

Keywords: Chronic Disease, Enzymes - genetics, Glutathione - biosynthesis, Glutathione - metabolism, Glutathione Peroxidase - metabolism, Lung - drug effects, Lung - injuries, Lung - metabolism, Lung - pathology, Nitrogen Dioxide - toxicity, Oxidation-Reduction - drug effects, Oxidative Stress - drug effects, RNA, Messenger - genetics, RNA, Messenger - metabolism, Chronic Disease, Enzymes - genetics, Glutathione - metabolism, Glutathione Peroxidase - metabolism, Lung - pathology, Lung Injury, Nitrogen Dioxide - toxicity, Oxidation-Reduction - drug effects, Oxidative Stress - drug effects, RNA, Messenger - metabolism

Add Comment 0 Comments

Editorial

01 November 2024 : Editorial  

Editorial: Artificial Intelligence (AI), Digital Image Analysis, and the Future of Cancer Diagnosis and Prognosis

Dinah V. Parums

DOI: 10.12659/MSM.947038

Med Sci Monit 2024; 30:e947038

0:00

In Press

Clinical Research  

Iliac Periosteal Bone Autografting vs Talus Non-Weight-Bearing Surgery in Hepple V Talus Osteochondral Inju...

Med Sci Monit In Press; DOI: 10.12659/MSM.944912  

Clinical Research  

Impact of Different Orthodontic Appliances on Mandibular Condyle, Anterior Region of Mandibular, and Maxill...

Med Sci Monit In Press; DOI: 10.12659/MSM.946265  

Clinical Research  

Risk Factors for Bone Cement Displacement After Percutaneous Kyphoplasty in Osteoporotic Vertebral Fracture...

Med Sci Monit In Press; DOI: 10.12659/MSM.945884  

Clinical Research  

Adipose-Derived Mesenchymal Stem Cells from Arthritis Patients: Differential Modulation of CD4⁺ T Cell Acti...

Med Sci Monit In Press; DOI: 10.12659/MSM.945273  

Most Viewed Current Articles

17 Jan 2024 : Review article   6,407,835

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,909,112

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   695,529

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

07 Jan 2022 : Meta-Analysis   260,637

Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...

DOI :10.12659/MSM.935074

Med Sci Monit 2022; 28:e935074

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750