18 June 2002
Human leptin administered intraperitoneally stimulates natriuresis and decreasesrenal medullary Na+, K+-ATPase activity in the rat - impaired effect in dietary-induced obesity.
Jerzy Bełtowski, Grazyna W jcicka, Dionizy Górny, Andrzej MarciniakMed Sci Monit 2002; 8(6): BR221-229 :: ID: 420820
Abstract
BACKGROUND: Leptin, produced by adipose tissue, apart from regulating foodintake and energy expenditure, also has natriuretic activity. In this study we examined the effect ofleptin on renal Na+, K+-ATPase responsible for active tubular sodium reabsorption, and compared the renaleffects of leptin in lean and obese rats.MATERIAL/METHODS: Male Wistar rats were either kept on normallaboratory chow or made obese by a high-calorie diet. The animals were placed in metabolic cages andurine was collected in 2-hour periods.RESULTS: In lean animals, leptin (1 mg/kg i.p.) caused a 139.5%increase in urine output, a 112.4% increase in natriuresis, and a 57.2% increase in the fractional excretionof sodium, but had no effect on the glomerular filtration rate. Leptin at this dose decreased renal medullaryNa+, K+-ATPase activity at 30 minutes, 1 hour and 2 hours by 31%, 34.3% and 21.2%, respectively. Theeffect of leptin on Na+, K+-ATPase at 1 hour was dose-dependent; the lowest dose inducing significantinhibition was 0.25 mg/kg. By contrast, leptin had no effect on either cortical Na+, K+-ATPase or theouabain-resistant fraction of ATPase. In obese rats, leptin increased urine output by only 29.1% andnatriuresis by 28.9%, and had no significant effect on medullary Na+, K+-ATPase.CONCLUSIONS: Leptin stimulatesnatriuresis primarily by inhibiting tubular sodium reabsorption. This effect is mediated, at least partially,by decreased Na+, K+-ATPase activity in the renal medulla, and is impaired in obese rats.
Keywords: Blood Pressure, Diet, Injections, Intraperitoneal, Kidney Medulla, Leptin, Na(+)-K(+)-Exchanging ATPase, Natriuresis, Obesity, Research Support, Non-U.S. Gov
Editorial
01 February 2025 : Editorial
Editorial: Current Approaches to Screening for Lung Cancer in Smokers and Non-SmokersDOI: 10.12659/MSM.948255
Med Sci Monit 2025; 31:e948255
In Press
Clinical Research
Pre- and Post-Surgical MRI Analysis of Levator Ani in Pelvic Organ Prolapse Patients: A Single-Center StudyMed Sci Monit In Press; DOI: 10.12659/MSM.945993
Clinical Research
Comparative Impact of Kinesio Taping and Post-Isometric Muscle Relaxation on Pain and Myofascial Mechanics ...Med Sci Monit In Press; DOI: 10.12659/MSM.945376
Clinical Research
Surgical Efficacy in Varicocele Ligation with Ephedrine-Assisted Blood Pressure ControlMed Sci Monit In Press; DOI: 10.12659/MSM.946234
Clinical Research
Retrospective Study to Compare Injury Patterns and Associations in 170 Patients Following Electric Scooter ...Med Sci Monit In Press; DOI: 10.12659/MSM.947155
Most Viewed Current Articles
17 Jan 2024 : Review article 6,968,277
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 701,791
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 25,442
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 20,020
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912