Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

18 June 2002

Human leptin administered intraperitoneally stimulates natriuresis and decreasesrenal medullary Na+, K+-ATPase activity in the rat - impaired effect in dietary-induced obesity.

Grazyna W jcicka, Jerzy Bełtowski, Dionizy Górny, Andrzej Marciniak

Med Sci Monit 2002; 8(6): BR221-229 :: ID: 420820


BACKGROUND: Leptin, produced by adipose tissue, apart from regulating foodintake and energy expenditure, also has natriuretic activity. In this study we examined the effect ofleptin on renal Na+, K+-ATPase responsible for active tubular sodium reabsorption, and compared the renaleffects of leptin in lean and obese rats.MATERIAL/METHODS: Male Wistar rats were either kept on normallaboratory chow or made obese by a high-calorie diet. The animals were placed in metabolic cages andurine was collected in 2-hour periods.RESULTS: In lean animals, leptin (1 mg/kg i.p.) caused a 139.5%increase in urine output, a 112.4% increase in natriuresis, and a 57.2% increase in the fractional excretionof sodium, but had no effect on the glomerular filtration rate. Leptin at this dose decreased renal medullaryNa+, K+-ATPase activity at 30 minutes, 1 hour and 2 hours by 31%, 34.3% and 21.2%, respectively. Theeffect of leptin on Na+, K+-ATPase at 1 hour was dose-dependent; the lowest dose inducing significantinhibition was 0.25 mg/kg. By contrast, leptin had no effect on either cortical Na+, K+-ATPase or theouabain-resistant fraction of ATPase. In obese rats, leptin increased urine output by only 29.1% andnatriuresis by 28.9%, and had no significant effect on medullary Na+, K+-ATPase.CONCLUSIONS: Leptin stimulatesnatriuresis primarily by inhibiting tubular sodium reabsorption. This effect is mediated, at least partially,by decreased Na+, K+-ATPase activity in the renal medulla, and is impaired in obese rats.

Keywords: Blood Pressure, Diet, Injections, Intraperitoneal, Kidney Medulla, Leptin, Na(+)-K(+)-Exchanging ATPase, Natriuresis, Obesity, Rats, Wistar, Research Support, Non-U.S. Gov

Add Comment 0 Comments


01 February 2024 : Editorial  

Editorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise

Dinah V. Parums

DOI: 10.12659/MSM.943911

Med Sci Monit 2024; 30:e943911


In Press

08 Feb 2024 : Clinical Research  

Predictors of Mortality in Veterans with Amyotrophic Lateral Sclerosis: Respiratory Status and Speech Disor...

Med Sci Monit In Press; DOI: 10.12659/MSM.943288  


25 Feb 2024 : Clinical Research  

Patterns and Outcomes of Traumatic Suicides: A Retrospective Study of 132 Patients Admitted to a Turkish Me...

Med Sci Monit In Press; DOI: 10.12659/MSM.943505  


01 Feb 2024 : Review article  

Review of the Evaluation of Pulmonary Hypoplasia as an Important Determinant of Clinical Outcomes in Infant...

Med Sci Monit In Press; DOI: 10.12659/MSM.943259  


19 Feb 2024 : Clinical Research  

Diagnostic Accuracy of Ileocolic Artery and Vein Diameter for Acute Appendicitis

Med Sci Monit In Press; DOI: 10.12659/MSM.943846  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750