22 February 2002
Distribution of hepatitis C virus RNA in whole blood of patients with HCV infectionand HBV-HCV coinfection.
Włodzimierz Mazur, Ilona Bednarek, Barbara Strzałka, Magdalena Jurzak, Tadeusz Wilczok, Urszula Mazurek, Ewa Laskowska-Lepiarczyk, Zbigniew GonciarzMed Sci Monit 2002; 8(2): CR125-130 :: ID: 420928
Abstract
BACKGROUND: Although HCV and HBV are essentially hepatoptropic, severallines of evidence suggest that these viruses can infect other cells, also PBMC in most patients withchronic HCV. MATERIAL/METHODS: The presence of HBV DNA and HCV-RNA was determined by a polymerase chainreaction (multiplex PCR and RT-PCR - nested PCR) in a group of patients with chronic liver disease. HCV-RNAwas investigated in serum, plasma and peripheral blood mononuclear cells (PBMC) while HBV-DNA only inserum or plasma. RESULTS: Among 374 patients tested, HCV-RNA was detected in the venous blood of 208patients; HCV RNA alone was detected in 154 patients and 54 patients were co-infected by HCV and HBV.HBV-DNA was found in 128 of 374 patients, while infection by HBV only was found in 74 patients. It wasalso shown that in the presence of HBV the replication ability of HCV is lower (p=0.085, Goodman-KruskalGamma = 0.561 and YuleQ = 0.5610). CONCLUSIONS: Since coexistence of HBV and HCV is not a rare case,diagnostics of hepatitis cannot be limited to detection of one type of the virus only. Misinterpretationof the virus type that caused the infection may lead to serious complications, especially in those caseswhen interferone is used for treatment.
Keywords: Base Sequence, DNA Primers, Hepacivirus, Hepatitis B, Hepatitis C, Polymerase Chain Reaction, RNA, Viral, Research Support, Non-U.S. Gov, Sensitivity and Specificity, Virus Replication
843 1
Editorial
01 December 2023 : Editorial
Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory InfectionsDOI: 10.12659/MSM.943312
Med Sci Monit 2023; 29:e943312
In Press
04 Dec 2023 : Animal Research
Effects of Intrathecal Ketamine on Cerebrospinal Fluid Levels of Brain-Derived Neurotrophic Factor and Mech...Med Sci Monit In Press; DOI:
01 Dec 2023 : Clinical Research
Risk Factors and Clinical Outcomes of COVID-19 Infection in Multiple Sclerosis Patients: A Retrospective St...Med Sci Monit In Press; DOI:
30 Nov 2023 : Review article
Decoding the Neurological Sequelae of General Anesthesia: A ReviewMed Sci Monit In Press; DOI:
30 Nov 2023 : Clinical Research
Enhanced Pain Relief and Muscle Growth in Individuals with Low Back Instability: The Impact of Blood Flow R...Med Sci Monit In Press; DOI:
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292