Logo Medical Science Monitor

Call: +1.631.470.9640
Closed: National Holiday

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

09 January 2002

Evaluation of the usefulness of the ELISA method for detection of enterovirus antibodies in serum samples of patients with myocarditis.

Włodzimierz Gut, Anna Wielkopolska, Iwona Binduga-Gajewska, Zdzisław Jarzabek

Med Sci Monit 2002; 8(1): MT10-14 :: ID: 420962


BACKGROUND: Enteroviruses are among the etiological factors in myocarditis.In the majority of cases it is impossible to obtain a direct confirmation of the presence of the virus,and typologically specific methods are of limited applicability, due both to the number of enterovirusesthat can cause myocarditis, and to the serological cross reactions among them. The goal of our researchwas to determine the usability of the ELISA test and the complement fixation reaction (CFR) in diagnostictesting of the serum of patients with myocarditis. MATERIAL/METHODS: The research material consistedof 117 serum samples collected from patients with myocarditis. In all these samples we measured the concentrationof IgG and IgM enterovirus antibodies using the ELISA method, and the height of the titer using the CFRmethod. RESULTS: The presence of IgM antibodies is a confirmation of current infection. A positive resultwas obtained for 61% of the patients. On the other hand, if one accepts as confirmation of infectiona titer significantly different from the level observed in the healthy population, namely > or =16 forCFR and > or =11 for ELISA-IgG (cf. positive results of 33% for CFR and 18.8% for ELISA IgG in our patients),the results may be disproportional. If one assumes as a test criterion a significant increase in titer,the presence of infection was confirmed in 28.5% of cases for CFR and 62% for ELISA-IgG. CONCLUSIONS:An analysis of the results obtained here justify the conclusion that neither the height of the CFR titernor the concentration of IgG antibodies constitutes a clear confirmation of infection, if the criterionof evaluation is based on a measure in a single serum sample. In this case it is necessary to test fora significant increase in the titer. The use of the average titer for the population at large to evaluateresults leads to both false positives and false negatives. Testing for IgM antibodies in a single serumsample is a faster method which confirms the current presence of infection. Our results indicate thatthe ELISA test can be used to diagnose myocarditis, since it is more sensitive and faster than CFR, andallows for the differentiation of antibody classes.

Keywords: Adolescent, Antibodies, Chemistry, Clinical, Enterovirus, Enzyme-Linked Immunosorbent Assay, Immunoglobulin G, Immunoglobulin M, myocarditis

Add Comment 0 Comments

594 4


01 May 2023 : Editorial  

Editorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.940911

Med Sci Monit 2023; 29:e940911

In Press

28 May 2023 : Database Analysis  

Scientometric and Visualized Analysis of Intravoxel Incoherent Motion Magnetic Resonance Imaging: 1988-2021

Med Sci Monit In Press; DOI: 10.12659/MSM.938715  

25 May 2023 : Database Analysis  

The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland

Med Sci Monit In Press; DOI: 10.12659/MSM.940904  

25 May 2023 : Review article  

A Review of Preclinical and Clinical Studies in Support of the Role of Non-Steroidal Anti-Inflammatory Drug...

Med Sci Monit In Press; DOI: 10.12659/MSM.940635  

25 May 2023 : Clinical Research  

Periapical Lesions and Missed Canals in Endodontically Treated Teeth: A Cone-Beam Computed Tomographic Stud...

Med Sci Monit In Press; DOI: 10.12659/MSM.940533  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750