01 February 2006
Establishing melanocyte cultures in a serum-free system for transplantation in vitiligo patients.
Rafał Czajkowski, Waldemar Placek, Tomasz Drewa, Dorota Olszewska-Słonina, Jan Sir, Bogna Kowaliszyn, Marcin WeissMed Sci Monit 2006; 12(2): CR63-69 :: ID: 445238
Abstract
Background: The aim of the study was to establish melanocyte cultures obtainedfrom the epidermis of vitiligo patients for future treatment and to estimate the influence of selectedfactors on the formation of suction blisters and the results of culture. Material/Methods: Twenty patientswith generalized vitiligo were qualified to the study. The melanocyte donor site was the arm or forearmwhere, using an electric vacuum suction machine and a plastic plate, suction blisters were formed. Theremoved roofs of the blisters were used as a melanocyte source for culture establishment. After an enzymaticbath, the cell suspension was transferred to culture flasks containing melanocyte growth medium (MGMM2). The melanocytes in culture were identified by immunocytochemistry. Results: Suction blisters wereformed in all patients. No melanocyte cultures were established from patients whose blisters appearedover a period 58 minutes. In addition, a suitable quantity of melanocytes was not obtained for eightother patients (with skin phototype II and/or without previous PUVA therapy). Conclusions: The epidermisof the arm and forearm is a good donor site for the establishment of melanocyte cultures. The effectivenessof culture depends mainly on the time of the formation of suction blisters, the phototype of the skin,and previous PUVA therapy.
Keywords: Arm, Cell Culture Techniques - methods, Cell Separation, Culture Media, Serum-Free, Epidermis - pathology, Melanocytes - transplantation, PUVA Therapy, Phenotype, Suction, Transplantation, Autologous, Vitiligo - surgery
627 26
Editorial
01 June 2023 : Editorial
Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic PreparednessDOI: 10.12659/MSM.941209
Med Sci Monit 2023; 29:e941209
In Press
05 Jun 2023 : Clinical Research
Comparison of Texture and Color Enhancement Imaging with White Light Imaging in 52 Patients with Short-Segm...Med Sci Monit In Press; DOI: 10.12659/MSM.940249
05 Jun 2023 : Laboratory Research
Comparison of Composite Resin (Duo-Shade) Shade Guide with Vita Ceramic Shades Before and After Chemical an...Med Sci Monit In Press; DOI: 10.12659/MSM.940949
02 Jun 2023 : Database Analysis
The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in PolandMed Sci Monit In Press; DOI: 10.12659/MSM.940904
01 Jun 2023 : Clinical Research
Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...Med Sci Monit In Press; DOI: 10.12659/MSM.940472
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952