Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 March 2005

Bifenthrin activates homotypic aggregation in human T-cell lines.

Nataly Hoffman, Van Tran, Anthony Daniyan, Olutosin Ojugbele, Stephen Pryor, Josephine Bonventre, Katherine Flynn, Benjamin Weeks

Med Sci Monit 2006; 12(3): BR87-94 :: ID: 447101


Background: Here, we addressed the concern that, despite the lack of overttoxicity, exposure to low levels of the common household pyrethroid pesticide, bifenthrin, could causeharm to the immune system. To do this, we measure the effect of bifenthrin on phytohemagglutinin (PHA)activation of homotypic aggregation in human T-cell lines. Material/Methods: The human CD4+ H9, and Jurkatcell lines and the human promonocyte U937 cell line, were exposed to varying concentrations of bifenthrin.Cell viability was determined using the AlmarBlue Toxicity Assay. Concentrations of bifenthrin whichdid not reduce cell viability were determined and these concentrations were tested for the effect ofbifenthrin on PHA-mediated homotypic aggregation. Blocking antibodies to ICAM and LFA-1 were used todisrupt aggregation and a nonspecific IgG was used as a control. Results: Bifenthrin was found to benontoxic at concentrations ranging from 10[sup]-4[/sup] to 10[sup]-13[/sup] M. Bifenthrin did not inhibit PHA induced cellaggregation in all cell lines tested. However, at 10[sup]-4[/sup] M, bifenthrin to form aggregates stimulatedhomotypic aggregation in the H9 and Jurkat T-cell lines. The bifenthrin-induced aggregate formation,like that seen with PHA, was blocked by treating the cells with antibodies to either LFA-1 or ICAM. Conclusions:The results here show that bifenthrin activates T-cell function by stimulating ICAM/LFA-1 mediated homotypicaggregation. This data suggests that exposure to bifenthrin, even at "acceptable" limits, can increasethe risk for and frequency of inflammatory responses and diseases such as asthma.

Keywords: Intercellular Adhesion Molecule-1 - immunology, U937 Cells, Time Factors, T-Lymphocytes - immunology, Pyrethrins - pharmacology, Phytohemagglutinins - pharmacology, Lymphocyte Function-Associated Antigen-1 - immunology, Jurkat Cells, Cell Aggregation - immunology, Insecticides - pharmacology, Hela Cells, Dose-Response Relationship, Drug, Cell Survival - drug effects, Antibodies, Monoclonal - metabolism

Add Comment 0 Comments

484 192


01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209


In Press

08 Jun 2023 : Clinical Research  

Risk Factors for New Vertebral Compression Fracture After Percutaneous Vertebral Augmentation: A Retrospect...

Med Sci Monit In Press; DOI: 10.12659/MSM.940134  

08 Jun 2023 : Clinical Research  

A Nomogram for Identifying HR+/Her2- Breast Cancer Patients with Positive Sentinel Lymph Nodes and Omitted ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940124  

08 Jun 2023 : Clinical Research  

Burden of COVID-19 on Mental Health of Resident Doctors in Poland

Med Sci Monit In Press; DOI: 10.12659/MSM.940208  

08 Jun 2023 : Clinical Research  

Risk Prediction for Rapidly Progressive Interstitial Lung Disease in Anti-MDA5-Positive Dermatomyositis: Th...

Med Sci Monit In Press; DOI: 10.12659/MSM.940251  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750