Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 May 2006

Ethanol abrogates Angiotensin II-stimulated vascular smooth muscle cell growth.

Monique L. Cain, Robert L. Hester, Ernest B. Izevbigie

Med Sci Monit 2006; 12(5): BR162-168 :: ID: 450281

Abstract

Background: Aberrant vascular smooth muscle cell (VSMC) proliferation isone of the etiological factors for hypertension and stroke. Angiotensin II (Ang II) and ethanol (EtOH)have been shown to modulate the proliferation of vascular smooth muscle cells (VSMCs) individually, butthe combined effects of Ang II and EtOH on VSMC proliferative activities are unknown. The objective ofthis study was to determine the effects of EtOH, Ang II, and the combination of Ang II and EtOH on DNAsynthesis, cell number, cyclic AMP (cAMP) production, and Mitogen-Activated Protein Kinase (MAPK) or(p44/42) activities in murine primary VSMCs. Material/Methods: Cell growth was determined by [[sup]3[/sup]H] thymidineincorporation and confirmed by cell counts using a hemacytometer. Cyclic AMP assays were carried outusing commercially available kits. MAPK activity was determined by immunoprecipitation studies usingan ELK-1 fusion protein as a substrate. Results: Ang II (10 microM) stimulated DNA synthesis by four-fold(P

Keywords: Angiotensin II - pharmacology, Cell Proliferation - drug effects, Cells, Cultured, Cyclic AMP - biosynthesis, DNA - biosynthesis, Drug Interactions, Enzyme Activation - drug effects, Ethanol - pharmacology, MAP Kinase Signaling System - drug effects, Mitogen-Activated Protein Kinase 1 - metabolism, Mitogen-Activated Protein Kinase 3 - metabolism, Muscle, Smooth, Vascular - metabolism

0 Comments

Editorial

01 July 2022 : Editorial  

Editorial: World Health Organization (WHO) Variants of Concern Lineages Under Monitoring (VOC-LUM) in Response to the Global Spread of Lineages and Sublineages of Omicron, or B.1.1.529, SARS-CoV-2

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.937676

Med Sci Monit 2022; 28:e937676

SARS-CoV-2/COVID-19

27 June 2022 : Clinical Research  

Shoulder Injury Related to Vaccine Administration (SIRVA) in 16 Patients Following COVID-19 Vaccination Who...

Med Sci Monit In Press; DOI: 10.12659/MSM.937430  

27 May 2022 : Clinical Research  

Factors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)

Med Sci Monit 2022; 28:e936547

In Press

05 Jul 2022 : Clinical Research  

Attention-Deficit/Hyperactivity Disorder (ADHD) and Time Perception in Adults: Do Adults with Different ADH...

Med Sci Monit In Press; DOI: 10.12659/MSM.936849  

01 Jul 2022 : Clinical Research  

Does Early-Period Patient Dissatisfaction Turn Into Satisfaction Over Time After Total Knee Replacement?

Med Sci Monit In Press; DOI: 10.12659/MSM.936792  

30 Jun 2022 : Laboratory Research  

Bcl-2 19-kDa Interacting Protein 3 (BNIP3)-Mediated Mitophagy Attenuates Intermittent Hypoxia-Induced Human...

Med Sci Monit In Press; DOI: 10.12659/MSM.936760  

30 Jun 2022 : Clinical Research  

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

Med Sci Monit In Press; DOI: 10.12659/MSM.936335  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750