Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 May 2006

The effect of preservatives on the sterility of microorganisms introduced into different fruit juices.

Basil C. Nzeako, Sheikha Al-Hashmi

Med Sci Monit 2006; 12(5): BR179-186 :: ID: 450284


Background: The aim of this study was to investigate the degree of sterilityof some fruit juices post manufacture and to determine how effective the preservatives in them aid thekilling of some microorganisms introduced into them. Material/Methods: Blackcurrant/raspberry, apple,guava, orange, mango, and mixed fruits were each checked for sterility by inoculating 100 microl separatelyin tryptone soy broth, Robertson's cooked meat, and Sabouraud broth and incubating for growth for 72hours at 37 degrees C. Twenty-milliliter aliquots of each juice were subsequently kept in a 37 degreesC water bath and inoculated separately with S. aureus, P. aeruginosa, E. coli, and C. albicans. At predeterminedtime intervals, a 1:250 dilution of each juice with the individual organism was made and the dilutionswere plated on blood and Sabouraud plates for viable counts. Each 1:250 dilution was then incubated alongwith the plates. The pH of each undiluted juice was determined. Results: All the juices were found tobe sterile. The growth inhibition exhibited by each juice was bactericidal or bacteriostatic, fungicidalor fungistatic, depending on the type of juice. The pH of the juices varied from 2.53-3.75. Conclusions:We found the juices to be bacteriologically free of organisms associated with food infection. Thoughthe juices were sterile, they could allow the survival of some bacteria and yeasts if these gained accessinto them. We found the orange, blackcurrant/raspberry, and mango juices to be bactericidal and fungicidal,while pineapple, mixed fruit, and guava were fungistatic, though with varying bactericidal activities.

Keywords: Candida albicans - growth & development, Beverages - microbiology, Antifungal Agents - pharmacology, Anti-Bacterial Agents - pharmacology, Colony Count, Microbial, Escherichia coli - growth & development, Food Microbiology, Food Preservatives - pharmacology, Fruit, Hydrogen-Ion Concentration, Oman, Pseudomonas aeruginosa - growth & development, Staphylococcus aureus - growth & development



04 June 2022 : Editorial  

Editorial: Acute Hepatitis of Unknown Origin in Children. Is Autoimmunity at Play?

Nahum Mendez-Sanchez, Shreya C. Pal
Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico

DOI: 10.12659/MSM.937371

Med Sci Monit 2022; 28:e937371


30 June 2022 : Editorial  

Editorial: World Health Organization (WHO) Variants of Concern Lineages Under Monitoring (VOC-LUM) in Respo...

Med Sci Monit In Press; DOI: 10.12659/MSM.937676  

27 June 2022 : Clinical Research  

Shoulder Injury Related to Vaccine Administration (SIRVA) in 16 Patients Following COVID-19 Vaccination Who...

Med Sci Monit In Press; DOI: 10.12659/MSM.937430  

In Press

30 Jun 2022 : Laboratory Research  

Bcl-2 19-kDa Interacting Protein 3 (BNIP3)-Mediated Mitophagy Attenuates Intermittent Hypoxia-Induced Human...

Med Sci Monit In Press; DOI: 10.12659/MSM.936760  

30 Jun 2022 : Editorial  

Editorial: World Health Organization (WHO) Variants of Concern Lineages Under Monitoring (VOC-LUM) in Respo...

Med Sci Monit In Press; DOI: 10.12659/MSM.937676  

30 Jun 2022 : Clinical Research  

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

Med Sci Monit In Press; DOI: 10.12659/MSM.936335  

28 Jun 2022 : Meta-Analysis  

Urodynamics in Early Diagnosis of Diabetic Bladder Dysfunction in Women: A Systematic Review and Meta-Analysis

Med Sci Monit In Press; DOI: 10.12659/MSM.937166  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750