01 May 2006
Urinary activities of cathepsin B, N-acetyl-ss-D-glucosaminidase, and albuminuriain patients with type 2 diabetes mellitus.
Agnieszka Piwowar, Maria Knapik-Kordecka, Izabela Fus, Maria WarwasMed Sci Monit 2006; 12(5): CR210-214 :: ID: 450290
Abstract
Background: The proximal kidney tubule contains a large number of lysosomesinvolved in the breakdown of intracellular as well as reabsorbed proteins. When tubular protein reabsorptionis overburdened or when there is tubular cell damage, higher urinary excretion of lysosomal enzymes,e.g. cathepsins and N-acetyl-beta-D-glucosaminidase (NAG), can be found. We compared urinary cathepsinB (CB) activity with NAG in diabetic patients. Material/Methods: Using fluorogenic substrates, urinaryand plasma CB and NAG activities in 130 type 2 diabetic patients with varying stages of albuminuria and42 control subjects were determined. Early morning urine samples were used. Results: In the patients,only higher values of plasma NAG were found. In urine, CB and NAG activities increased progressivelyfrom normoalbuminuria, through microalbuminuria to macroalbuminuria group. The normoalbuminuria grouphad both enzyme activities higher than healthy controls. Urine CB activity in the patients also increasedgradually to tertiles of urinary NAG. Only urinary CB activity was significantly associated with glycemicstate. The correlation was stronger in the patients with poor glycemic control. The plasma/urine ratiosfor both CB and NAG decreased in the patients compared with controls. Conclusions: Determination of urinaryCB activity might be useful as a non-invasive surrogate marker of incipient nephropathy.
Keywords: Acetylglucosaminidase - urine, Aged, 80 and over, Albuminuria - urine, Biological Markers - urine, Case-Control Studies, Cathepsin B - urine, Diabetes Mellitus, Type 2 - urine, Diabetic Nephropathies - urine
820 12
Editorial
01 December 2023 : Editorial
Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory InfectionsDOI: 10.12659/MSM.943312
Med Sci Monit 2023; 29:e943312
In Press
04 Dec 2023 : Animal Research
Effects of Intrathecal Ketamine on Cerebrospinal Fluid Levels of Brain-Derived Neurotrophic Factor and Mech...Med Sci Monit In Press; DOI:
01 Dec 2023 : Clinical Research
Risk Factors and Clinical Outcomes of COVID-19 Infection in Multiple Sclerosis Patients: A Retrospective St...Med Sci Monit In Press; DOI:
30 Nov 2023 : Review article
Decoding the Neurological Sequelae of General Anesthesia: A ReviewMed Sci Monit In Press; DOI:
30 Nov 2023 : Clinical Research
Enhanced Pain Relief and Muscle Growth in Individuals with Low Back Instability: The Impact of Blood Flow R...Med Sci Monit In Press; DOI:
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292