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01 June 2006

Two stage EDTA anti-calcification method for bioprosthetic heart valve materials.

Nehir Sucu, Kerem Karaca, Nejat Yilmaz, Nejat Yilmaz, Barlas Aytacoglu, Lulufer Tamer, Murat Ozeren, H. Ali Dondas, Yzzet Oguz, Oya Ogenler, Murat Dikmengil

Med Sci Monit 2006; 12(6): MT33-38 :: ID: 451275


Background: The aim of this study was to investigate the effect of two-stageEDTA treatment in diminishing calcific degeneration in bovine pericardial bioprosthetic heart valve material.Material/Methods: Conventionally preserved pericardium specimens were divided into two groups. GroupI (controls, n=18) pieces were first fixed in phosphate-buffered solution (PBS)+0.6% glutaraldehyde at+4 degrees C for 24 hours, then stored in PBS+0.2% glutaraldehyde at room temperature for 6 days. GroupII (study group, n=18) pieces were treated with PBS containing 100 microg/ml ethylenediaminetetraaceticacid (EDTA) at +4 degrees C for 24 hours, then fixed in PBS+0.6% glutaraldehyde as was group I at +4degrees C for 24 hours. After a second exposure to PBS containing 100 microg/ml EDTA at room temperaturefor 24 hours, they were stored in PBS+0.2% glutaraldehyde at room temperature for 4 days. Pericardialpatches were inserted into the dorsal pouches of 18 juvenile male Wistar rats. After 7 weeks of implantation,all the pericardium pieces were harvested from sacrificed rats. The calcium content and biomechanicalproperties of the explanted tissues were evaluated and also examined histopathologically. Results: Thedifference in the calcium content of the control and study groups was statistically significant. Biomechanicaland histopathologic assessment also supported these findings. Conclusions: Application of two-stage EDTAwas found to be useful in the attenuation of calcification in bioprosthetic heart valve materials withmildly increased durability. As calcification was reduced by approximately 50%, it can be consideredfor use with other agents as an adjuvant treatment.

Keywords: Bioprosthesis, Calcinosis - prevention & control, Calcium - analysis, Cattle, Edetic Acid - pharmacology, Heart Valve Prosthesis, Heart Valve Prosthesis Implantation, Pericardium - ultrastructure, Tissue Preservation - methods

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Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.943312

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750