01 June 2006
Two stage EDTA anti-calcification method for bioprosthetic heart valve materials.Nehir Sucu, Kerem Karaca, Nejat Yilmaz, Nejat Yilmaz, Barlas Aytacoglu, Lulufer Tamer, Murat Ozeren, H. Ali Dondas, Yzzet Oguz, Oya Ogenler, Murat Dikmengil
Med Sci Monit 2006; 12(6): MT33-38 :: ID: 451275
Background: The aim of this study was to investigate the effect of two-stageEDTA treatment in diminishing calcific degeneration in bovine pericardial bioprosthetic heart valve material.Material/Methods: Conventionally preserved pericardium specimens were divided into two groups. GroupI (controls, n=18) pieces were first fixed in phosphate-buffered solution (PBS)+0.6% glutaraldehyde at+4 degrees C for 24 hours, then stored in PBS+0.2% glutaraldehyde at room temperature for 6 days. GroupII (study group, n=18) pieces were treated with PBS containing 100 microg/ml ethylenediaminetetraaceticacid (EDTA) at +4 degrees C for 24 hours, then fixed in PBS+0.6% glutaraldehyde as was group I at +4degrees C for 24 hours. After a second exposure to PBS containing 100 microg/ml EDTA at room temperaturefor 24 hours, they were stored in PBS+0.2% glutaraldehyde at room temperature for 4 days. Pericardialpatches were inserted into the dorsal pouches of 18 juvenile male Wistar rats. After 7 weeks of implantation,all the pericardium pieces were harvested from sacrificed rats. The calcium content and biomechanicalproperties of the explanted tissues were evaluated and also examined histopathologically. Results: Thedifference in the calcium content of the control and study groups was statistically significant. Biomechanicaland histopathologic assessment also supported these findings. Conclusions: Application of two-stage EDTAwas found to be useful in the attenuation of calcification in bioprosthetic heart valve materials withmildly increased durability. As calcification was reduced by approximately 50%, it can be consideredfor use with other agents as an adjuvant treatment.
Keywords: Bioprosthesis, Calcinosis - prevention & control, Calcium - analysis, Cattle, Edetic Acid - pharmacology, Heart Valve Prosthesis, Heart Valve Prosthesis Implantation, Pericardium - ultrastructure, Tissue Preservation - methods
01 May 2023 : EditorialEditorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy
Med Sci Monit 2023; 29:e940911
31 May 2023 : Review articlePrevalence, Diagnosis, and Management of Eclampsia and the Need for Improved Maternal Care: A Review
Med Sci Monit In Press; DOI: 10.12659/MSM.939919
30 May 2023 : Clinical ResearchA Case-Control Study of Cognitive Function in Patients with End-Stage Renal Disease Before and After Hemodi...
Med Sci Monit In Press; DOI: 10.12659/MSM.940409
30 May 2023 : Review articleA Review of the Role of the Zebrafish (Danio reiro) in Preclinical and Clinical Models of Biomarker Identif...
Med Sci Monit In Press; DOI: 10.12659/MSM.940550
30 May 2023 : Database AnalysisThe COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland
Med Sci Monit In Press; DOI: 10.12659/MSM.940904
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952