Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

28 January 2003

The influence of etoposide on the distribution of tubulin in human leukemia cell line HL-60.

Alina Grzanka, Dariusz Grzanka

Med Sci Monit 2003; 9(1): BR66-69 :: ID: 4781


BACKGROUND: In the last decade there has been increasing interest in cytoskeletal research in relation to important process of cell biology. However, after decades of debate many issues remain unsettled. The aim of our study was to estimate the distribution of tubulin in etoposide-induced HL-60 cells and address the question of whether the reorganization of tubulin may be involved in apoptosis. MATERIAL/METHODS: HL-60 cells were incubated with etoposide (0.02-200 mM/L) for 72 hours. Tubulin was estimated by the indirect immunofluorescence method. TRITC-labeled goat anti rabbit IgG was used to label tubulin. RESULTS: Significant changes occurred in cell morphology of and tubulin organization at 200 mM/L etoposide: large cells, brightly labeled for tubulin, in cytoplasm. At 200 uM/L etoposide, we also observed an increase in apoptotic cells with bright staining for tubulin at the site of apoptotic body formations. Cells with features of apoptosis were frequently seen also at 20 mM/L etoposide. The exposure of cells to 0.02, 0.2, or 2 mM/L etoposide did not result in any visible changes in morphology or tubulin organization. Cell size, the number of viable cells, and the percentage of apoptotic cells correlated significantly with the dose of etoposide (p<0.001). CONCLUSIONS: These results indicate that etoposide promotes changes in the distribution of tubulin in HL-60 cells, depending on the dose, and that the reorganization of tubulin seems to be involved in apoptosis

Keywords: Antineoplastic Agents, Phytogenic - pharmacology, Cell Division - drug effects, Dose-Response Relationship, Drug, Etoposide - pharmacology, Fluorescent Antibody Technique, Indirect, HL-60 Cells, Leukemia - drug therapy, Tubulin - metabolism, Tumor Cells, Cultured

Add Comment 0 Comments


01 June 2024 : Editorial  

Editorial: Concerns as Highly Pathogenic Avian Influenza (HPAI) Virus of the H5N1 Subtype is Identified in Dairy Cows and Other Mammals

Dinah V. Parums

DOI: 10.12659/MSM.945315

Med Sci Monit 2024; 30:e945315


In Press

Clinical Research  

Epidemiology and Risk Factors for Revision Total Knee Arthroplasty in Chinese Patients: A Retrospective Stu...

Med Sci Monit In Press; DOI: 10.12659/MSM.943681  

Clinical Research  

Effects of the Cold Pressor Test on Popliteal Vein Diameter, Flow Velocity, and Blood Flow in the Lower Lim...

Med Sci Monit In Press; DOI: 10.12659/MSM.944560  

Clinical Research  

Outcomes of Anterior Lateral Approach and Gerdy's Tubercle Osteotomy for Tibial Plateau Fractures Involving...

Med Sci Monit In Press; DOI: 10.12659/MSM.943946  

Clinical Research  

Improving Function and Quality of Life in Patients with Chronic Neck Pain, Tension-Type Headache, and Forwa...

Med Sci Monit In Press; DOI: 10.12659/MSM.944315  

Most Viewed Current Articles

14 Dec 2022 : Clinical Research   1,494,766

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


17 Jan 2024 : Review article   1,476,009

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


16 May 2023 : Clinical Research   690,201

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial   50,159

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750