We studied 160 normal consecutive singleton pregnancies presenting between January 2004 and June 2005 , all of whom underwent amniocentesis for fetal karyotype from 16 to 19 weeks of gestation (mean 17.4 weeks). The data were divided into four gestational age groups : (1) 40 at 16 weeks, (2) 40 at 17 weeks, (3) 40 at 18 weeks, and (4) 40 at 19 weeks of gestation). Serum Copper (Cu) levels were determined by atomic absorption spectrophotometer . Serum calcium (Ca) , phosphorus, magnesium (Mg), iron levels were measured using clinical chemistry kits. Statistical analyses were performed by using One-Way Anova (Post Hoc Bonferroni) test. A value of p<0.05 was considered statistically significant. This is a twenty two years old male patient with weight loss, abdominal pain, hepatomegaly and elevated liver function tests. The serological markers for viral B, C hepatitis and tumoral markers were normal. The CT scan demonstrated a hipodense, nodular lesion in the liver and the histological examination was reported as a typical fibrolamellar hepatocarcinoma.

Keywords: heart rate variability, time domain analysis, frequency domain analysis, Risk Factors