Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 May 1997

Studies on the structural background on the cross-inhibition of the products in the arginine metabolism of macrophages

Andras Hrabak, Tamas Bajor, Agnes Temesi, Gyorgy Meszaros

Med Sci Monit 1997; 3(3): BR299-304 :: ID: 500773


Arginase and nitric oxide synthase (NOS) were inhibited by nitrite and putrescine, respectively. Results showed a cross-inhibition by the products of the two arginine utilizing pathways in macrophages. The kinetics of these inhibitions have been described earlier. Arginase was measured by the release of urea; NOS activity was determined by measuring 14C-L-citrulline synthesis from 14C-labeled L-arginine. The structural changes of arginase were studied by fluorescence and gel filtration experiments. Nitrite caused a decrease of tryptophane fluorescence over 5 mM concentration without dissociating the arginase oligomers as indicated by gel filtration experiments. The differences in the structural features of L-arginine substrate and putrescine inhibitor made the binding of putrescine to the active site of NOS unlikely. In conclusion, our studies suggest that nitrite causes a non-competitive inhibition of arginase based on a conformational change without the dissociation of the arginase oligomers. For putrescine inhibition we suggest an allosteric mechanism also based on a conformational change.

Keywords: macrophage, Arginase, NO synthase, Fluorescence, Gel Filtration, Conformational change

Add Comment 0 Comments

395 0


01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209


In Press

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

01 Jun 2023 : Clinical Research  

Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.940873  

01 Jun 2023 : Clinical Research  

Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.940187  

01 Jun 2023 : Clinical Research  

Influence of Insulin Resistance on Diabetes Nephropathy

Med Sci Monit In Press; DOI: 10.12659/MSM.939482  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750