Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 July 1997

Modulation of surface platelet membrane receptors in patients with coronary artery disease during cardiopulmonary bypass - a preliminary report

Ryszard Golański, Jacek Golański, Cezary Watała, Krzysztof Chiżyński, Sławomir Chrul, Jolanta Zochniak, Alicja Iwaszkiewicz

Med Sci Monit 1997; 3(4): SR437-445 :: ID: 501488


The nature of the supposed effects of cardiopulmonary bypass (CPB) on defects in platelet function remains controversial, however, cardiac surgery is believed to be associated with a disruption of the haemostatic system. We employed whole blood flow cytometry to evaluate the possible alterations in the expression of platelet surface membrane glycoproteins in resting and thrombin- or ADP-activated platelets in peripheral blood. As monitored by the use of monoclonal antibodies directed against glycoproteins GPIb, GPIIIa, GPIIbIIIa complexes and P-selectin, circulating blood platelets subjected to extracorporeal circulation were characterized by: a/ time-dependent increase in platelet surface P-selectin, b/ paralleled marked reductions in GPIb and GPIIIa, c/ increased microparticle fraction with no platelet clumping. In comparison to circulating blood platelets collected during CPB, the alterations in platelet P-selectin in post-surgery blood (20 h after bypass) were negligible. We observed the loss in platelet reactivity at every time point of CPB, both in response to time-driven spontaneous anticoagulant-induced activation and following the in vitro action of thrombin and ADP; these depressions occurred with respect to all the monitored platelet surface antigens and remained least marked in post-surgery blood.Our results seem to furnish support for a novel outlook on the molecular mechanisms of the altered expression of platelet surface membrane receptors during CPB. We suggest that (1) markedly deficient platelet reactivity in response to agonist-induced platelet activation, (2) loss of platelet surface membrane glycoproteins GPIb and GPIIIa, (3) enhanced expression of surface membrane P-selectin, and (4) increased number of platelet microparticles point to platelet increased consumption/rupture, degranulation, membrane protein shedding, or all of the above effects as major contributors to altered expression of platelet membrane receptors in CPB patients. Noteworthy, a markedly depressed number/expression of platelet fibrinogen receptor, which, in turn, became augmented in post-surgery blood, implies that CPB can make peripheral blood platelets more vulnerable to pro-aggregatory action of agonists in vivo. This last observation seems critical since it validates the suspicion of the enhanced risk for early occlusion of vein bypass grafts in patients undergoing coronary artery surgery.

Keywords: Cardiopulmonary Bypass, Coronary Artery Disease, platelet receptors, Platelet Activation, platelet consumption/rupture, release reaction, adhesion receptors

Add Comment 0 Comments

415 1


01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209


In Press

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

01 Jun 2023 : Clinical Research  

Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.940873  

01 Jun 2023 : Clinical Research  

Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.940187  

01 Jun 2023 : Clinical Research  

Influence of Insulin Resistance on Diabetes Nephropathy

Med Sci Monit In Press; DOI: 10.12659/MSM.939482  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750