Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 September 1998

Attempt to evaluate activation of peripheral blood lymphocytes (CD69) after gluten challenge in children with coeliac disease

Andrzej Marek, Maria Korzon, Tomasz Smiatacz, Jacek Brodzicki

Med Sci Monit 1998; 4(5): CR821-825 :: ID: 502018


Both the phenomenon of activating immune system of alimentary tract (GALT- gut associated lymphoid tissue) after gluten challenge as well as the role of cellular immune response in the development of morphological changes of intestinal mucosa in children with coeliac disease are well known. The introduction of new laboratory techniques and flow cytometry in particular, as well as the use of monoclonal antibodies opened new possibilities in the studies upon correlation between processes taking place in GALT and the changes within peripheral blood lymphocytes. At the same time, a question emerged whether these studies will allow to reduce the frequency of biopsy of intestinal mucosa in children with malabsorption syndrome, especially after gluten challenge. The aim of our study was to assess the diagnostic value of changes in the expression of selected CD antigens on T cells in peripheral blood as a result of gluten challenge. The intensification of CD3, CD19 and CD69 expression on peripheral blood lymphocytes was investigated before and after introducing gluten into the diet of 13 children with coeliac disease and of 7 children with secondary coeliac syndrome. Monoclonal antibodies and flow cytometry were used. The results obtained varied greatly in both groups. It was found that T cells activation prevails in children with coeliac disease, while in the case of children with secondary coeliac syndrome, the tendency for B cells activation is dominant. These differences, however, are not statistically significant. It is likely that the inflow of T and B cells with CD69 expression to circulation which is intensified by gluten challenge, is compensated by equally intensive homing of the lymphocytes in the mucous membrane of alimentary tract. Thus, the assessment of the intensification of CD69 expression on T and B cells of peripheral blood is useful neither in the differentiation between coeliac disease and secondary malabsorption syndrome, nor in the determination of disease intensity.

Keywords: peripheral blood lymphocytes, CD69 antigen, coeliac disease, children

Add Comment 0 Comments

556 0


01 December 2023 : Editorial  

Editorial: Outbreaks of Post-Pandemic Childhood Pneumonia and the Re-Emergence of Endemic Respiratory Infections

Dinah V. Parums

DOI: 10.12659/MSM.943312

Med Sci Monit 2023; 29:e943312


In Press

08 Dec 2023 : Clinical Research  

Association Between Influenza Vaccine Uptake and Health Awareness: A Cross-Sectional Questionnaire-Based St...

Med Sci Monit In Press; DOI:  

04 Dec 2023 : Animal Research  

Effects of Intrathecal Ketamine on Cerebrospinal Fluid Levels of Brain-Derived Neurotrophic Factor and Mech...

Med Sci Monit In Press; DOI:  

01 Dec 2023 : Clinical Research  

Risk Factors and Clinical Outcomes of COVID-19 Infection in Multiple Sclerosis Patients: A Retrospective St...

Med Sci Monit In Press; DOI:  

30 Nov 2023 : Review article  

Decoding the Neurological Sequelae of General Anesthesia: A Review

Med Sci Monit In Press; DOI:  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788


30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750