01 October 2007
Absorption rates and free radical scavenging values of vitamin C-lipid metabolites in human lymphoblastic cellsBenjamin S Weeks, Pedro P Perez
Med Sci Monit 2007; 13(10): BR205-210 :: ID: 502324
Background: In this study we investigated the cellular absorption rates, antioxidant and free radical scavenging activity of vitamin C-lipid metabolites. The absorption was measured in a human lymphoblastic cell line using a spectrophotometric technique.
Material/Methods: Cellular vitamin C levels in the human lymphoblastic H9 cell line were measured using the 2,4-dinitrophenylhydrazine spectrophotometric technique. Free radical scavenging activity of vitamin C-lipid metabolites was measured by the reduction of 1,1-diphenyl-2-picryl hydrazyl (DPPH) to 1,1-diphenyl-2-picryl hydrazine. Vitamin C-lipid metabolite scavenging of peroxyl radical oxygen reactive species (ORAC) was determined by fluorescence spectrophotometry.
Results: Compared to ascorbic acid (AA), calcium ascorbate (CaA), and calcium ascorbate-calcium threonate-dehydroascorbate (Ester-C®), vitamin C-lipid metabolites (PureWay-C™) were more rapidly absorbed by the H9 human T-lymphocytes. The vitamin C-lipid metabolites (PureWay-C™) also reduced pesticide-induced T-lymphocyte aggregation by 84%, while calcium ascorbate-calcium threonate-dehydroascorbate (Ester-C®) reduced aggregation by only 34%. The vitamin C-lipid metabolites (PureWay-C™) demonstrated free radical scavenging activity of nearly 100% reduction of DPPH at 20 µg/ml and oxygen radical scavenging of over 1200µ Trolox® equivalents per gram.
Conclusions: These data demonstrate that the vitamin C-lipid metabolites (PureWay-C™) are more rapidly taken-up and absorbed by cells than other forms of vitamin C, including Ester-C®. This increased rate of absorption correlates with an increased protection of the T-lymphocytes from pesticide toxicities. Further, vitamin C-lipid metabolites (PureWay-C™) are a potent antioxidant and have significant free radical scavenging capabilities.
Keywords: Ascorbic Acid - metabolism, Antioxidants - metabolism, Absorption, Cell Line, Diet, Free Radical Scavengers - metabolism, Lipid Metabolism, Reactive Oxygen Species - metabolism, T-Lymphocytes - metabolism, Xenobiotics - metabolism
01 June 2023 : EditorialEditorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness
Med Sci Monit 2023; 29:e941209
07 Jun 2023 : Review articleUltrasound-Guided Brachial Plexus Block by Costoclavicular Space Approach: A Narrative Review
Med Sci Monit In Press; DOI: 10.12659/MSM.939920
05 Jun 2023 : Clinical ResearchComparison of Texture and Color Enhancement Imaging with White Light Imaging in 52 Patients with Short-Segm...
Med Sci Monit In Press; DOI: 10.12659/MSM.940249
05 Jun 2023 : Laboratory ResearchComparison of Composite Resin (Duo-Shade) Shade Guide with Vita Ceramic Shades Before and After Chemical an...
Med Sci Monit In Press; DOI: 10.12659/MSM.940949
02 Jun 2023 : Database AnalysisThe COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland
Med Sci Monit In Press; DOI: 10.12659/MSM.940904
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952